Dietary lipids coinduce xenobiotic metabolizing enzymes in rat liver

Eun K. Yang, Anna Radominska, Bruce S. Winder, Andrew J. Dannenberg

    Research output: Contribution to journalArticlepeer-review

    37 Scopus citations

    Abstract

    We examined the role of dietary lipids in regulating the activities and amounts of epoxide hydrolase, UDP-glucuronosy-syltransferase and glutathione S-transferase in rat liver. Male Wistar rats were fed a fat-free (FF) diet or isocaloric control diet containing 5% corn oil (CO) or 5% fish oil (FO) for 3 weeks. The activities of these enzymes were approx. 2-fold higher in rats fed the FO diet vs. the FF diet. Intermediate levels of enzyme activity were found in rats fed the CO diet. Diet-induced differences in enzyme levels were shown by immunoblotting. The highest levels of epoxide hydrolase, UDP-glucuronosy-syltransferase and glutathione S-transferase were detected in rats fed the FO diet. The lowest levels of these enzymes were found in rats fed the FF diet. Intermediate levels of enzyme were found in rats fed the CO diet. Thus, diet-induced differences in enzyme activities were paralleled by changes in enzyme levels. Fatty acid analysis of microsomal lipids showed that the FF diet was associated with decreased levels of n - 6 fatty acids vs. the CO diet. The FO diet resulted in increased levels of n - 3 fatty acids vs. the other diets.

    Original languageEnglish (US)
    Pages (from-to)52-58
    Number of pages7
    JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
    Volume1168
    Issue number1
    DOIs
    StatePublished - May 20 1993

    Keywords

    • (Rat)
    • Epoxide hydrolase
    • Glutathione S-transferase
    • Liver
    • UDP-glucuronosyltransferase

    ASJC Scopus subject areas

    • Endocrinology
    • Biophysics
    • Biochemistry

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