DHX9 pairs with IPS-1 to sense double-stranded RNA in myeloid dendritic cells

Zhiqiang Zhang, Bin Yuan, Ning Lu, Valeria Facchinetti, Yong Jun Liu

Research output: Contribution to journalArticle

111 Scopus citations

Abstract

The innate immune system is equipped with many molecular sensors for microbial DNA/RNA to quickly mount antimicrobial host immune responses. In this paper, we identified DHX9, a DExDc helicase family member, as an important viral dsRNA sensor in myeloid dendritic cells (mDCs). Knockdown of DHX9 expression by small heteroduplex RNA dramatically blocked the ability of mDCs to produce IFN-αβ and proinflammatory cytokines in response to polyinosine-polycytidylic acid, influenza A, and reovirus. DHX9 could specifically bind polyinosine-polycytidylic acid via its double-strand RNA binding motifs. DHX9 interacted with IPS- 1 via the HelicC-HA2-DUF and CARD domains of DHX9 and IPS-1, respectively. Knockdown of DHX9 expression in mDCs blocked the activation of NF-κB and IFN regulatory factor 3 by dsRNA. Collectively, these results suggest that DHX9 is an important RNA sensor that is dependent on IPS-1 to sense pathogenic RNA.

Original languageEnglish (US)
Pages (from-to)4501-4508
Number of pages8
JournalJournal of Immunology
Volume187
Issue number9
DOIs
StatePublished - Nov 1 2011

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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