Developmental changes in the amount and functional state of UDP-glucuronosyltransferase

Andrew J. Dannenberg; Howard J. Worman; Suzanne Scarlata

doi:10.1016/0304-4165(92)90036-T

Developmental changes in the amount and functional state of UDP-glucuronosyltransferase

Andrew J. Dannenberg, Howard J. Worman, Suzanne Scarlata

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The effect of postnatal development on the activity of liver microsomal UDP-glucuronosyltransferase was determined in male Wistar rats between 25 and 200 days of age using p-nitrophenol as aglycone. Enzyme activity (measured at 1.0 mM UDP-glucuronic acid, 0.05 mM p-nitrophenol) decreased 55% between 25 and 88 days of age and was constant thereafter. Treatment of microsomes with palmitoyl-lysophosphatidylcholine, which allows for an estimation of the amount of enzyme, showed approximately a four-fold decrease in enzyme concentration during the same period. This decrease was confirmed by Western blotting of microsomes with anti-UDP-glucuronosyltransferase antiserum. The fact that a nearly four-fold decline in enzyme concentration led to only a 55 decrease in activity indicates that there was an increase in activity per molecule of UDP-glucuronosyltransferase as the concentration of enzyme decreased. Treatment of microsomes with high pressure or detergent caused a greater extent of enzyme activation in microsomes prepared from 25 than 200 day old rats, suggesting that a fraction of the enzyme in older rats was activated in untreated microsomes. Fatty acid analysis of liver microsomal lipids during postnatal development revealed changes in docosahexaenoic acid (22:6) which correlated with levels of UDP-glucuronosyltransferase activity.

Original language	English (US)
Pages (from-to)	250-255
Number of pages	6
Journal	BBA - General Subjects
Volume	1116
Issue number	3
DOIs	https://doi.org/10.1016/0304-4165(92)90036-T
State	Published - Jun 12 1992

Keywords

(Rat)
Development
Liver
UDP-glucuronosyltransferase

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry

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10.1016/0304-4165(92)90036-T

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title = "Developmental changes in the amount and functional state of UDP-glucuronosyltransferase",

abstract = "The effect of postnatal development on the activity of liver microsomal UDP-glucuronosyltransferase was determined in male Wistar rats between 25 and 200 days of age using p-nitrophenol as aglycone. Enzyme activity (measured at 1.0 mM UDP-glucuronic acid, 0.05 mM p-nitrophenol) decreased 55% between 25 and 88 days of age and was constant thereafter. Treatment of microsomes with palmitoyl-lysophosphatidylcholine, which allows for an estimation of the amount of enzyme, showed approximately a four-fold decrease in enzyme concentration during the same period. This decrease was confirmed by Western blotting of microsomes with anti-UDP-glucuronosyltransferase antiserum. The fact that a nearly four-fold decline in enzyme concentration led to only a 55 decrease in activity indicates that there was an increase in activity per molecule of UDP-glucuronosyltransferase as the concentration of enzyme decreased. Treatment of microsomes with high pressure or detergent caused a greater extent of enzyme activation in microsomes prepared from 25 than 200 day old rats, suggesting that a fraction of the enzyme in older rats was activated in untreated microsomes. Fatty acid analysis of liver microsomal lipids during postnatal development revealed changes in docosahexaenoic acid (22:6) which correlated with levels of UDP-glucuronosyltransferase activity.",

keywords = "(Rat), Development, Liver, UDP-glucuronosyltransferase",

author = "Dannenberg, {Andrew J.} and Worman, {Howard J.} and Suzanne Scarlata",

note = "Funding Information: This work was supportedb y grants from the NIH (IK08 DKI992. KIIDK01790, GM3t)9241 and NSF (DMB8504014)A. .D. is the recipiento f the Dr. Mark WeiusteinL iver Scholar Award. The authorst hankDr. Thomas Donnclly lot his assistancein preparingt he anti-UDP-glucuronus3'ltransferaanstei serumW. e also thank Mr. Murray Cohen for his assistancei n the course of this research.",

year = "1992",

month = jun,

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doi = "10.1016/0304-4165(92)90036-T",

language = "English (US)",

volume = "1116",

pages = "250--255",

journal = "BBA - General Subjects",

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T1 - Developmental changes in the amount and functional state of UDP-glucuronosyltransferase

AU - Dannenberg, Andrew J.

AU - Worman, Howard J.

AU - Scarlata, Suzanne

N1 - Funding Information: This work was supportedb y grants from the NIH (IK08 DKI992. KIIDK01790, GM3t)9241 and NSF (DMB8504014)A. .D. is the recipiento f the Dr. Mark WeiusteinL iver Scholar Award. The authorst hankDr. Thomas Donnclly lot his assistancein preparingt he anti-UDP-glucuronus3'ltransferaanstei serumW. e also thank Mr. Murray Cohen for his assistancei n the course of this research.

PY - 1992/6/12

Y1 - 1992/6/12

N2 - The effect of postnatal development on the activity of liver microsomal UDP-glucuronosyltransferase was determined in male Wistar rats between 25 and 200 days of age using p-nitrophenol as aglycone. Enzyme activity (measured at 1.0 mM UDP-glucuronic acid, 0.05 mM p-nitrophenol) decreased 55% between 25 and 88 days of age and was constant thereafter. Treatment of microsomes with palmitoyl-lysophosphatidylcholine, which allows for an estimation of the amount of enzyme, showed approximately a four-fold decrease in enzyme concentration during the same period. This decrease was confirmed by Western blotting of microsomes with anti-UDP-glucuronosyltransferase antiserum. The fact that a nearly four-fold decline in enzyme concentration led to only a 55 decrease in activity indicates that there was an increase in activity per molecule of UDP-glucuronosyltransferase as the concentration of enzyme decreased. Treatment of microsomes with high pressure or detergent caused a greater extent of enzyme activation in microsomes prepared from 25 than 200 day old rats, suggesting that a fraction of the enzyme in older rats was activated in untreated microsomes. Fatty acid analysis of liver microsomal lipids during postnatal development revealed changes in docosahexaenoic acid (22:6) which correlated with levels of UDP-glucuronosyltransferase activity.

AB - The effect of postnatal development on the activity of liver microsomal UDP-glucuronosyltransferase was determined in male Wistar rats between 25 and 200 days of age using p-nitrophenol as aglycone. Enzyme activity (measured at 1.0 mM UDP-glucuronic acid, 0.05 mM p-nitrophenol) decreased 55% between 25 and 88 days of age and was constant thereafter. Treatment of microsomes with palmitoyl-lysophosphatidylcholine, which allows for an estimation of the amount of enzyme, showed approximately a four-fold decrease in enzyme concentration during the same period. This decrease was confirmed by Western blotting of microsomes with anti-UDP-glucuronosyltransferase antiserum. The fact that a nearly four-fold decline in enzyme concentration led to only a 55 decrease in activity indicates that there was an increase in activity per molecule of UDP-glucuronosyltransferase as the concentration of enzyme decreased. Treatment of microsomes with high pressure or detergent caused a greater extent of enzyme activation in microsomes prepared from 25 than 200 day old rats, suggesting that a fraction of the enzyme in older rats was activated in untreated microsomes. Fatty acid analysis of liver microsomal lipids during postnatal development revealed changes in docosahexaenoic acid (22:6) which correlated with levels of UDP-glucuronosyltransferase activity.

KW - (Rat)

KW - Development

KW - Liver

KW - UDP-glucuronosyltransferase

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U2 - 10.1016/0304-4165(92)90036-T

DO - 10.1016/0304-4165(92)90036-T

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SN - 0304-4165

VL - 1116

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JO - BBA - General Subjects

JF - BBA - General Subjects

IS - 3

ER -

Developmental changes in the amount and functional state of UDP-glucuronosyltransferase

Abstract

Keywords

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