TY - JOUR
T1 - Development of denervation alterations in surface membranes of mammalian skeletal muscle
AU - Smith, P. Blaise
AU - Appel, S. H.
N1 - Funding Information:
1 This work was supported by Grant MH 08394 from the National Institute of Mental Health and Grants NS 07872 and NS 12213 from the National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health. Dr. Smith’s present address is Department of Biochemistry, The Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1977/7
Y1 - 1977/7
N2 - The effect of denervation on selected biochemical properties of skeletal muscle surface membranes was studied. Studies were performed on muscle denervated 1, 2, 3, 5, or 7 days. After denervation the surface membranes of skeletal muscle underwent specific biochemical alterations without gross changes in membrane polypeptide or lipid composition. Seven days after denervation sarcolemmal membranes underwent a slight density shift. Na+,K+(Mg2+)-ATPase activity and sialic acid content increased beginning 3 days postdenervation. Basal, sodium fluoride-stimulated, and isoproterenolstimulated adenyl cyclase activity decreased 50% after 5 days of denervation. Isoproterenol-stimulated activity was enhanced by 5′-guanylyl imidodiphosphate [Gpp (NH) p]. Membrane tentatively identified as transverse tubule increased in yield as denervation progressed. In this fraction endogenous protein kinase activity for a 28,000-MW polypeptide decreased by 50% between days 2 and 3. The decrease in membrane protein phosphorylation was paralleled by a decrease in content of the 28,000-MW polypeptide. These membrane changes may help contribute to our understanding of the physiological alterations observed in skeletal muscles after denervation.
AB - The effect of denervation on selected biochemical properties of skeletal muscle surface membranes was studied. Studies were performed on muscle denervated 1, 2, 3, 5, or 7 days. After denervation the surface membranes of skeletal muscle underwent specific biochemical alterations without gross changes in membrane polypeptide or lipid composition. Seven days after denervation sarcolemmal membranes underwent a slight density shift. Na+,K+(Mg2+)-ATPase activity and sialic acid content increased beginning 3 days postdenervation. Basal, sodium fluoride-stimulated, and isoproterenolstimulated adenyl cyclase activity decreased 50% after 5 days of denervation. Isoproterenol-stimulated activity was enhanced by 5′-guanylyl imidodiphosphate [Gpp (NH) p]. Membrane tentatively identified as transverse tubule increased in yield as denervation progressed. In this fraction endogenous protein kinase activity for a 28,000-MW polypeptide decreased by 50% between days 2 and 3. The decrease in membrane protein phosphorylation was paralleled by a decrease in content of the 28,000-MW polypeptide. These membrane changes may help contribute to our understanding of the physiological alterations observed in skeletal muscles after denervation.
UR - https://www.scopus.com/pages/publications/0017739803
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U2 - 10.1016/0014-4886(77)90142-X
DO - 10.1016/0014-4886(77)90142-X
M3 - Article
C2 - 140807
AN - SCOPUS:0017739803
SN - 0014-4886
VL - 56
SP - 102
EP - 114
JO - Experimental Neurology
JF - Experimental Neurology
IS - 1
ER -