Development of denervation alterations in surface membranes of mammalian skeletal muscle

P. Blaise Smith, Stanley H. Appel

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The effect of denervation on selected biochemical properties of skeletal muscle surface membranes was studied. Studies were performed on muscle denervated 1, 2, 3, 5, or 7 days. After denervation the surface membranes of skeletal muscle underwent specific biochemical alterations without gross changes in membrane polypeptide or lipid composition. Seven days after denervation sarcolemmal membranes underwent a slight density shift. Na+,K+(Mg2+)-ATPase activity and sialic acid content increased beginning 3 days postdenervation. Basal, sodium fluoride-stimulated, and isoproterenolstimulated adenyl cyclase activity decreased 50% after 5 days of denervation. Isoproterenol-stimulated activity was enhanced by 5′-guanylyl imidodiphosphate [Gpp (NH) p]. Membrane tentatively identified as transverse tubule increased in yield as denervation progressed. In this fraction endogenous protein kinase activity for a 28,000-MW polypeptide decreased by 50% between days 2 and 3. The decrease in membrane protein phosphorylation was paralleled by a decrease in content of the 28,000-MW polypeptide. These membrane changes may help contribute to our understanding of the physiological alterations observed in skeletal muscles after denervation.

Original languageEnglish (US)
Pages (from-to)102-114
Number of pages13
JournalExperimental Neurology
Volume56
Issue number1
DOIs
StatePublished - Jan 1 1977

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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