Determination of Vitreous, Aqueous, and Plasma Concentration of Orally Administered Voriconazole in Humans

Seenu M. Hariprasad, William F. Mieler, Eric Holz, Hua Gao, Judy E. Kim, Jingduan Chi, Randall A. Prince

Research output: Contribution to journalArticle

243 Scopus citations

Abstract

Objective: To investigate the penetration of voriconazole, a new-generation triazole antifungal agent, into the vitreous and aqueous humor after oral administration. Methods: A prospective, nonrandomized clinical study included 14 patients scheduled for elective pars plana vitrectomy surgery between December 1, 2002, and February 28, 2003, at the Cullen Eye Institute, Houston, Tex. Aqueous, vitreous, and plasma samples were obtained and analyzed from 14 patients after oral administration of two 400-mg doses of voriconazole taken 12 hours apart before surgery. Assays were performed by means of highperformance liquid chromatography. Results: Mean±SD voriconazole concentrations in plasma (n = 14), vitreous (n = 14), and aqueous (n = 11) were 2.13±0.93 pg/mL, 0.81±0.31 pg/mL, and 1.13±0.57 pg/mL, respectively. Mean±SD sampling times after oral administration of the second voriconazole dose for plasma, vitreous, and aqueous were 2.4±0.6 hours, 3.0±0.5 hours, and 2.9±0.5 hours, respectively. The percentages of plasma voriconazole concentration achieved in the vitreous and aqueous were 38.1% and 53.0%, respectively. Mean vitreous and aqueous minimum inhibitory concentrations for 90% of isolates (MIC90) were achieved against a wide spectrum of yeasts and molds, including Aspergillus species and Candida species, along with many other organisms. Conclusions: Orally administered voriconazole achieves therapeutic aqueous and vitreous levels in the noninflamed human eye, and the activity spectrum appears to appropriately encompass the most frequently encountered mycotic species involved in the various causes of fungal endophthalmitis. Because of its broad spectrum of coverage, low MIC90 levels for the organisms of concern, good tolerability, and excellent bioavailability with oral administration, it may represent a major advance in the prophylaxis or management of exogenous or endogenous fungal endophthalmitis.

Original languageEnglish (US)
Pages (from-to)42-47
Number of pages6
JournalArchives of Ophthalmology
Volume122
Issue number1
DOIs
StatePublished - Jan 2004

ASJC Scopus subject areas

  • Ophthalmology

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