Determination of tannase activity by color plate method

Guangjun Nie, Jinshan Diao, Hui Liu, Li Wang, Zhiming Zheng

Research output: Contribution to journalArticle


In order to make an audio-visual, batch and accurate assay of tannase activity, color plate method was applied with tannic acid and bromocresol green as substrate and chromogenic agent, respectively. The concentrations of tannic acid, bromocresol green and agar, addition of tannase and reaction time were studied and analyzed. The results showed that the 1% of tannic acid, 0.001% bromocresol green, 3% agar, 30 μL tannase addition and 24 hours at 40 °C for reaction were the optimum for the plate method. The corresponding adjustable R square of standard curve was 98%. Verification experiment was carried out and its result shows that this method is accurate and stable for assaying tannase. Fig 7, Tab 1, Ref 11.

Original languageEnglish (US)
Pages (from-to)883-887
Number of pages5
JournalChinese Journal of Applied and Environmental Biology
Issue number6
StatePublished - Dec 25 2010


  • Agar
  • Bromocresol green
  • Color plate method
  • Tannic acid

ASJC Scopus subject areas

  • Pollution
  • Genetics
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

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