Determination of quantum dots in single cells by inductively coupled plasma mass spectrometry

Ling Na Zheng; Meng Wang; Bing Wang; Han Qing Chen; Ouyang Hong; Yu Liang Zhao; Zhi Fang Chai; Wei Yue Feng

doi:10.1016/j.talanta.2013.07.075

Determination of quantum dots in single cells by inductively coupled plasma mass spectrometry

Ling Na Zheng, Meng Wang, Bing Wang, Han Qing Chen, Ouyang Hong, Yu Liang Zhao, Zhi Fang Chai, Wei Yue Feng

Houston Methodist

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

In order to assess cytotoxicity of quantum dots (QDs), new reliable analytical techniques that can provide comparative information at a single-cell level are required. In this study, a single cell ICP-MS (SC-ICP-MS) method was established to determine intracellular QDs in single cells after exposure. Uptake kinetics of QDs into cells was studied using the established method. The results were compared and validated by flow cytometry and cell digestion methods. In contrast to other methods, SC-ICP-MS can directly detect QDs and their degradation products via elements, and thus is a promising complement to available methods for single cell analysis and is expected to be a critical tool in the future.

Original language	English (US)
Pages (from-to)	782-787
Number of pages	6
Journal	Talanta
Volume	116
DOIs	https://doi.org/10.1016/j.talanta.2013.07.075
State	Published - 2013

Keywords

ICP-MS
Nanotoxicity
Quantum dots
Single cell analysis

ASJC Scopus subject areas

General Chemistry

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10.1016/j.talanta.2013.07.075

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@article{b089205e50e54258bba580961eead473,

title = "Determination of quantum dots in single cells by inductively coupled plasma mass spectrometry",

abstract = "In order to assess cytotoxicity of quantum dots (QDs), new reliable analytical techniques that can provide comparative information at a single-cell level are required. In this study, a single cell ICP-MS (SC-ICP-MS) method was established to determine intracellular QDs in single cells after exposure. Uptake kinetics of QDs into cells was studied using the established method. The results were compared and validated by flow cytometry and cell digestion methods. In contrast to other methods, SC-ICP-MS can directly detect QDs and their degradation products via elements, and thus is a promising complement to available methods for single cell analysis and is expected to be a critical tool in the future.",

keywords = "ICP-MS, Nanotoxicity, Quantum dots, Single cell analysis",

author = "Zheng, {Ling Na} and Meng Wang and Bing Wang and Chen, {Han Qing} and Ouyang Hong and Zhao, {Yu Liang} and Chai, {Zhi Fang} and Feng, {Wei Yue}",

note = "Funding Information: This work was supported by the National Basic Research Program (973 Program: 2011CB933403 and 2012CB930000 ), the National Natural Science Foundation of China ( 21175136, 11275214 and 10975148 ), and NHMRC Training Fellowship . ",

year = "2013",

doi = "10.1016/j.talanta.2013.07.075",

language = "English (US)",

volume = "116",

pages = "782--787",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Determination of quantum dots in single cells by inductively coupled plasma mass spectrometry

AU - Zheng, Ling Na

AU - Wang, Meng

AU - Wang, Bing

AU - Chen, Han Qing

AU - Hong, Ouyang

AU - Zhao, Yu Liang

AU - Chai, Zhi Fang

AU - Feng, Wei Yue

N1 - Funding Information: This work was supported by the National Basic Research Program (973 Program: 2011CB933403 and 2012CB930000 ), the National Natural Science Foundation of China ( 21175136, 11275214 and 10975148 ), and NHMRC Training Fellowship .

PY - 2013

Y1 - 2013

N2 - In order to assess cytotoxicity of quantum dots (QDs), new reliable analytical techniques that can provide comparative information at a single-cell level are required. In this study, a single cell ICP-MS (SC-ICP-MS) method was established to determine intracellular QDs in single cells after exposure. Uptake kinetics of QDs into cells was studied using the established method. The results were compared and validated by flow cytometry and cell digestion methods. In contrast to other methods, SC-ICP-MS can directly detect QDs and their degradation products via elements, and thus is a promising complement to available methods for single cell analysis and is expected to be a critical tool in the future.

AB - In order to assess cytotoxicity of quantum dots (QDs), new reliable analytical techniques that can provide comparative information at a single-cell level are required. In this study, a single cell ICP-MS (SC-ICP-MS) method was established to determine intracellular QDs in single cells after exposure. Uptake kinetics of QDs into cells was studied using the established method. The results were compared and validated by flow cytometry and cell digestion methods. In contrast to other methods, SC-ICP-MS can directly detect QDs and their degradation products via elements, and thus is a promising complement to available methods for single cell analysis and is expected to be a critical tool in the future.

KW - ICP-MS

KW - Nanotoxicity

KW - Quantum dots

KW - Single cell analysis

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U2 - 10.1016/j.talanta.2013.07.075

DO - 10.1016/j.talanta.2013.07.075

M3 - Article

C2 - 24148474

AN - SCOPUS:84883171960

SN - 0039-9140

VL - 116

SP - 782

EP - 787

JO - Talanta

JF - Talanta

ER -

Determination of quantum dots in single cells by inductively coupled plasma mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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