Determination of inorganic and methyl mercury in biological samples by high performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry

Meng Wang, Weiyue Feng, Fang Zhang, Bing Wang, Junwen Shi, Bai Li, Zhifang Chai, Yuliang Zhao

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

High performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) has been developed for the determination of inorganic and methyl mercury in biological samples. Different techniques used to extract inorganic and methyl mercury from biological samples were also evaluated. The samples were sonicated to extract inorganic and methyl mercury in the system of 5 mol/L HCl. A 5% (V/V) methanol-water mobile phase containing 0.06 mol/L ammonium acetate, 20 μg/L Bi and 0.1% (V/V) 2-mercaptoethanol and a C 18 reversed-phase column (5 μm, 3.9 mm x 150 mm) was used for separation of CH 3Hg + and Hg 2+ by HPLC. The concentrations of CH 3Hg + and Hg 2+ in eluent were detected by ICP-MS. Total four certified reference materials including human hair (GBW 07601), prawn (GBW 08572) , fish (IAEA MA-B-3/TM) and bovine liver (GBW 080193) were analyzed. The results showed good accordance with the certified values. The detection limits of the method for inorganic and methyl mercury were 0.3 μg/L and 0.2 μg/L, respectively.

Original languageEnglish (US)
Pages (from-to)1671-1675
Number of pages5
JournalChinese Journal of Analytical Chemistry
Volume33
Issue number12
StatePublished - Dec 2005

Keywords

  • Biological samples
  • High performance liquid chromatography
  • Hyphened technique
  • Inductively coupled plasma-mass spectrometry
  • Mercury
  • Speciation analysis

ASJC Scopus subject areas

  • Analytical Chemistry

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