Determination of cysteine on low-density lipoproteins using the fluorescent probe, 5-iodoacetamidofluoresceine

Richard D. Coleman; Tae W. Kim; Antonio M. Gotto; Chao yuh Yang

doi:10.1016/0167-4838(90)90111-R

Determination of cysteine on low-density lipoproteins using the fluorescent probe, 5-iodoacetamidofluoresceine

Richard D. Coleman, Tae W. Kim, Antonio M. Gotto, Chao yuh Yang

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Using the fluorescent sulfhydryl probe, 5-iodoacetamidofluoresceine, to label the free sulfhydryl of low-density lipoprotein, the positions of two cysteine residues in apolipoprotein B were located. The tryptic peptides containing the fluorescent probe were isolated by high-performance liquid chromatography systems and sequenced by automatic techniques. The free cysteine residues of apolipoprotein B-100 on low-density lipoprotein are located at positions 3734 and 4190, either or both of which can potentially form a disulfide linkage with apolipoprotein(a) in lipoprotein(a).

Original language	English (US)
Pages (from-to)	129-132
Number of pages	4
Journal	Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume	1037
Issue number	1
DOIs	https://doi.org/10.1016/0167-4838(90)90111-R
State	Published - Jan 19 1990

Keywords

Amino acid sequence
Apolipoprotein B-100
Fluorescent labeling
Lipoprotein (a)
Low density lipoproteins
Sulfhydryl peptide

ASJC Scopus subject areas

Structural Biology
Molecular Biology
Biophysics
Biochemistry

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10.1016/0167-4838(90)90111-R

Cite this

@article{cee271ca020e4afbb4958a15b830ab48,

title = "Determination of cysteine on low-density lipoproteins using the fluorescent probe, 5-iodoacetamidofluoresceine",

abstract = "Using the fluorescent sulfhydryl probe, 5-iodoacetamidofluoresceine, to label the free sulfhydryl of low-density lipoprotein, the positions of two cysteine residues in apolipoprotein B were located. The tryptic peptides containing the fluorescent probe were isolated by high-performance liquid chromatography systems and sequenced by automatic techniques. The free cysteine residues of apolipoprotein B-100 on low-density lipoprotein are located at positions 3734 and 4190, either or both of which can potentially form a disulfide linkage with apolipoprotein(a) in lipoprotein(a).",

keywords = "Amino acid sequence, Apolipoprotein B-100, Fluorescent labeling, Lipoprotein (a), Low density lipoproteins, Sulfhydryl peptide",

author = "Coleman, \{Richard D.\} and Kim, \{Tae W.\} and Gotto, \{Antonio M.\} and Yang, \{Chao yuh\}",

note = "Funding Information: We thank B. Touchstone and M.L. Yang for excellent technical assistance, S. Kelly for the artwork, and M. Needham for preparation of this manuscript. This work was supported by a grant from the National Institutes of Health (National Research and Demonstration Center in Arteriosclerosis, HL-27341), a grant- in-aid from the American Heart Association (No. 870863), and a grant from The Methodist Hospital. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1990",

month = jan,

day = "19",

doi = "10.1016/0167-4838(90)90111-R",

language = "English (US)",

volume = "1037",

pages = "129--132",

journal = "Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular",

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T1 - Determination of cysteine on low-density lipoproteins using the fluorescent probe, 5-iodoacetamidofluoresceine

AU - Coleman, Richard D.

AU - Kim, Tae W.

AU - Gotto, Antonio M.

AU - Yang, Chao yuh

N1 - Funding Information: We thank B. Touchstone and M.L. Yang for excellent technical assistance, S. Kelly for the artwork, and M. Needham for preparation of this manuscript. This work was supported by a grant from the National Institutes of Health (National Research and Demonstration Center in Arteriosclerosis, HL-27341), a grant- in-aid from the American Heart Association (No. 870863), and a grant from The Methodist Hospital. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1990/1/19

Y1 - 1990/1/19

N2 - Using the fluorescent sulfhydryl probe, 5-iodoacetamidofluoresceine, to label the free sulfhydryl of low-density lipoprotein, the positions of two cysteine residues in apolipoprotein B were located. The tryptic peptides containing the fluorescent probe were isolated by high-performance liquid chromatography systems and sequenced by automatic techniques. The free cysteine residues of apolipoprotein B-100 on low-density lipoprotein are located at positions 3734 and 4190, either or both of which can potentially form a disulfide linkage with apolipoprotein(a) in lipoprotein(a).

AB - Using the fluorescent sulfhydryl probe, 5-iodoacetamidofluoresceine, to label the free sulfhydryl of low-density lipoprotein, the positions of two cysteine residues in apolipoprotein B were located. The tryptic peptides containing the fluorescent probe were isolated by high-performance liquid chromatography systems and sequenced by automatic techniques. The free cysteine residues of apolipoprotein B-100 on low-density lipoprotein are located at positions 3734 and 4190, either or both of which can potentially form a disulfide linkage with apolipoprotein(a) in lipoprotein(a).

KW - Amino acid sequence

KW - Apolipoprotein B-100

KW - Fluorescent labeling

KW - Lipoprotein (a)

KW - Low density lipoproteins

KW - Sulfhydryl peptide

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DO - 10.1016/0167-4838(90)90111-R

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AN - SCOPUS:0025139919

SN - 0167-4838

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JO - Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular

JF - Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular

IS - 1

ER -

Determination of cysteine on low-density lipoproteins using the fluorescent probe, 5-iodoacetamidofluoresceine

Abstract

Keywords

ASJC Scopus subject areas

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