Determination of cysteine on low-density lipoproteins using the fluorescent probe, 5-iodoacetamidofluoresceine

Richard D. Coleman, Tae W. Kim, Antonio Gotto, Chao yuh Yang

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Using the fluorescent sulfhydryl probe, 5-iodoacetamidofluoresceine, to label the free sulfhydryl of low-density lipoprotein, the positions of two cysteine residues in apolipoprotein B were located. The tryptic peptides containing the fluorescent probe were isolated by high-performance liquid chromatography systems and sequenced by automatic techniques. The free cysteine residues of apolipoprotein B-100 on low-density lipoprotein are located at positions 3734 and 4190, either or both of which can potentially form a disulfide linkage with apolipoprotein(a) in lipoprotein(a).

Original languageEnglish (US)
Pages (from-to)129-132
Number of pages4
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume1037
Issue number1
DOIs
StatePublished - Jan 19 1990

Keywords

  • Amino acid sequence
  • Apolipoprotein B-100
  • Fluorescent labeling
  • Lipoprotein (a)
  • Low density lipoproteins
  • Sulfhydryl peptide

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology
  • Biophysics
  • Biochemistry

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