TY - JOUR
T1 - Determination of 2,3,7,8-TCDD toxic equivalent factors (TEFs)
T2 - support for the use of the in vitro AHH induction assay
AU - Safe, S.
N1 - Funding Information:
ACKNOWLEDGEMENTS Several colleagues have collaborated on this project, and I would like to thank T. Sawyer, O. Hutzinger, B. Keys, G. Mason, M. Kelley, J. Piskorska-Pliszczynska, L. Safe, B. Zmudzka, K. Farrell, R. Bannister, J.A. Madge, B. Chittim, M.A. Denomme, K. Homonko, M.A. Li, B. Leece and R. Towner for their contributions. The financial assistance of the Texas Agricultural Experiment Station, the United States Environmental Protection Agency, the National Institutes of Health and the Center for Energy and Mineral Resources has made this work possible.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1987
Y1 - 1987
N2 - The in vitro induction of the cytochrome P1-450-dependent monooxygenases, aryl hydrocarbon hydroxylase (AHH) or ethoxyresorufin O-deethylase (EROD) by 2,3,7,8-TCDD and related toxic halogenated aryl hydrocarbons in rat hepatoma H-4-II E cells has been developed as a short term quantitative bioassay for these toxic chemicals. There was a linear correlation between the -log EC50 (in vitro) AHH induction vs the -log ED50 (in vivo) for body weight loss, thymic atrophy, hepatic AHH and EROD induction in the rat for several polychlorinated biphenyl, dibenzo-p-dioxin and dibenzofuran congeners and mixtures. These data clearly support the utility of the in vitro AHH induction assay as a short term test system for quantitating the "toxic or 2,3,7,8-TCDD equivalents" in an extract containing toxic halogenated aromatics. The bioassay method is rapid, relatively accurate and much more cost effective than conventional analytical methods such as gas-chromatography-mass spectrometry from which it is difficult to determine the levels of 2,3,7,8-TCDD equivalents in specific analytes.
AB - The in vitro induction of the cytochrome P1-450-dependent monooxygenases, aryl hydrocarbon hydroxylase (AHH) or ethoxyresorufin O-deethylase (EROD) by 2,3,7,8-TCDD and related toxic halogenated aryl hydrocarbons in rat hepatoma H-4-II E cells has been developed as a short term quantitative bioassay for these toxic chemicals. There was a linear correlation between the -log EC50 (in vitro) AHH induction vs the -log ED50 (in vivo) for body weight loss, thymic atrophy, hepatic AHH and EROD induction in the rat for several polychlorinated biphenyl, dibenzo-p-dioxin and dibenzofuran congeners and mixtures. These data clearly support the utility of the in vitro AHH induction assay as a short term test system for quantitating the "toxic or 2,3,7,8-TCDD equivalents" in an extract containing toxic halogenated aromatics. The bioassay method is rapid, relatively accurate and much more cost effective than conventional analytical methods such as gas-chromatography-mass spectrometry from which it is difficult to determine the levels of 2,3,7,8-TCDD equivalents in specific analytes.
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U2 - 10.1016/0045-6535(87)90012-9
DO - 10.1016/0045-6535(87)90012-9
M3 - Article
AN - SCOPUS:0023158397
SN - 0045-6535
VL - 16
SP - 791
EP - 802
JO - Chemosphere
JF - Chemosphere
IS - 4
ER -