Abstract
Wild-type Neurospora crassa is completely inhibited by 5 ppm nystatin. Ultraviolet-induced mutants have been isolated that grow in the presence of 60 ppm of the antibiotic. Gas-liquid chromatographic, mass spectroscopic, and nuclear magnetic resonance analyses showed the wild-type sterols to be ergosterol (ergosta-5,7,22-trien-3β-ol) and episterol (ergosta-7,24(28)-dien-3β-ol) in a 3:1 ratio. The mutants contained lichesterol (ergosta-5,8,22-trien-3β-ol) and fecosterol (ergosta-8,24(28)-dien-3β-ol) in a 2:1 ratio, differing from the wild type only in the position of the B-ring unsaturation. A deficiency of an ergosta-8,24 (28)-dien-3β-ol:ergosta-7,24(28)-dien-3β-ol isomerase is indicated.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 459-466 |
| Number of pages | 8 |
| Journal | Biochemical Genetics |
| Volume | 12 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 1974 |
Keywords
- lichesterol
- mutants
- Neurospora
- nystatin
- steroids
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Biochemistry
- Molecular Biology
- Genetics