TY - JOUR
T1 - Detection of the 2,3,7,8-tetpachlorodibenzo-p-dioxin (TCDD) receptor in rat liver by isoelectric focusing in polyacrylamide gels
AU - Carlstedt-Duke, Jan
AU - Elfström, Gunilla
AU - Snochowski, Marek
AU - Högberg, Bertil
AU - Gustafsson, Jan åke
N1 - Funding Information:
We are very grateful to Dr. A. Poland for his kind gift of [ 1,6-3H] and TCDBF. This work was supported by grants from Arbetarskyddsfonden, Research Foundation and Riksfijreningen mot Cancer.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1978/12
Y1 - 1978/12
N2 - A stereospecific, high-affinity binding protein (receptor protein) for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in rat liver cytosol can be demonstrated using isoelectric focusing in polyacrylamide gels. In its native form, the receptor protein focuses at a varying pI possibly due to aggregation. However, if limited proteolysis of the receptor is carried out, the trypsinized receptor focuses as a single sharp peak at pI 5.15-5.25. Using this method the receptor can be separated from a serum TCDD-binding protein (pI 5.7-5.8) that is resistant to dextran-coated charcoal (DCC) treatment. The binding of [3H] TCDD to the receptor is competed for by 2,3,7,8-tetrachlorodibenzofuran (TCDBF), 3-methylcholanthrene (MC), and {amalgamation}-naphthoflavone ({amalgamation}NF) but not by phenobarbital (PhB) or pregnenolone-16α-carbonitrile (PCN). The method described can be used for detection and quantitation of the TCDD receptor.
AB - A stereospecific, high-affinity binding protein (receptor protein) for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in rat liver cytosol can be demonstrated using isoelectric focusing in polyacrylamide gels. In its native form, the receptor protein focuses at a varying pI possibly due to aggregation. However, if limited proteolysis of the receptor is carried out, the trypsinized receptor focuses as a single sharp peak at pI 5.15-5.25. Using this method the receptor can be separated from a serum TCDD-binding protein (pI 5.7-5.8) that is resistant to dextran-coated charcoal (DCC) treatment. The binding of [3H] TCDD to the receptor is competed for by 2,3,7,8-tetrachlorodibenzofuran (TCDBF), 3-methylcholanthrene (MC), and {amalgamation}-naphthoflavone ({amalgamation}NF) but not by phenobarbital (PhB) or pregnenolone-16α-carbonitrile (PCN). The method described can be used for detection and quantitation of the TCDD receptor.
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U2 - 10.1016/0378-4274(78)90041-3
DO - 10.1016/0378-4274(78)90041-3
M3 - Article
AN - SCOPUS:0018078044
SN - 0378-4274
VL - 2
SP - 365
EP - 373
JO - Toxicology Letters
JF - Toxicology Letters
IS - 6
ER -