Detection of specific binding of [1,6-3H]2,3,7,8-tetrachlorodibenzo-p-dioxin ([3H]TCDD) in human leukocytes using electrofocusing in polyacrylamide gel

Mikael Gillner, Lars Arne Haldosén, Sven A. Gustafsson, Jan Åke Gustafsson

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The binding of [1,6-3H]2,3,7,8-tetrachlorodibenzo-p-dioxin ([3H]TCDD) in human leukocyte cytosol has been studied using electrofocusing in polyacrylamide gel. One single peak of bound [3H]TCDD is found after completed focusing with an isoelectric point of 6.0. The binding capacity for 1.5 nM [3H]TCDD in the leukocyte cytosol was completely saturated by incubation in the presence of 150 nM unlabelled 2,3,7,8-tetrachlorodibenzofuran. [3H]TCDD also binds to a single binding species in human serum and plasma which focuses at pH 5.2 and which is not saturated by the addition of a 100-fold excess of unlabelled 2,3,7,8-tetrachlorodibenzofuran to the incubation. The levels of specific binding of [3H]TCDD in leukocytes from healthy blood donors ranged from 0 to 50 fmol/mg cytosolic protein.

Original languageEnglish (US)
Pages (from-to)41-51
Number of pages11
JournalToxicology Letters
Volume47
Issue number1
DOIs
StatePublished - Apr 1989

Keywords

  • Electrofocusing
  • Lymphocytes
  • TCDD receptor

ASJC Scopus subject areas

  • Toxicology

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