Detection of prostate specific antigen with nanomechanical resonators

Philip S. Waggoner, Madhukar Varshney, Harold G. Craighead

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

In this work, we use arrays of nanomechanical resonators to detect prostate specific antigen (PSA), a protein biomarker associated with prostate cancer. The surfaces of our very thin, trampoline-like devices are functionalised for immunospecific capture of PSA molecules, and the mass of bound material can be detected as a reduction in the resonant frequency. Fetal bovine serum was spiked with known concentrations of PSA, and in conjunction with a nanoparticle-based sandwich assay, concentrations as low as 50 fg mL-1, or 1.5 fM, could be detected from the realistic samples. The presence of non-specific proteins in the serum did not significantly affect the sensitivity of our assay.

Original languageEnglish (US)
Pages (from-to)3095-3099
Number of pages5
JournalLab on a Chip
Volume9
Issue number21
DOIs
StatePublished - Jan 1 2009

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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