Detection of genomic alterations in human endometrial cancer by two-dimensional gel electrophoresis.

Susan Pohlod-Miller, James Fanning, Ping Gu, Keith A. Crist, Ming You

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

OBJECTIVE: The purpose of this study was to detect genomic alterations in human endometrial cancer by two-dimensional gel electrophoresis. STUDY DESIGN: With use of a newly developed two-dimensional gel electrophoresis assay, we scanned 19 high-risk DNA fragments for alterations in human endometrial hyperplasias and adenocarcinomas. This method includes cleaving of high-molecular-weight DNA, radioactive labeling, and separating DNA fragments by two-dimensional gel electrophoresis. By comparing the two-dimensional gel electrophoresis profile (spots) of neoplastic with normal endometrium, genetic alterations such as amplification, allelic loss, and hypermethylation or hypomethylation can be detected. RESULTS: Seven of 8 human endometrial adenocarcinoma (88%) and 1 of 2 hyperplasias (50%) revealed changes in spot density. The number of spots changed per specimen was 4. The median percentage of specimens with changes in an individual spot was 30%. Eleven spots had a reduction or loss of spot density, and 8 spots had an increase in spot density. CONCLUSION: By use of a novel two-dimensional gel electrophoresis assay, we identified genetic alterations in 50% of hyperplasias and 88% of endometrial adenocarcinomas.

Original languageEnglish (US)
Pages (from-to)855-857
Number of pages3
JournalAmerican Journal of Obstetrics and Gynecology
Volume186
Issue number5
DOIs
StatePublished - May 2002

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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