Detection of genetic alterations in mouse lung adenocarcinomas by two-dimensional gel electrophoresis

J. Liu; P. Gu; G. Bergman; G. J. Kelloff; C. W. Boone; M. You; Y. Wang

doi:10.1080/01902140150216738

Detection of genetic alterations in mouse lung adenocarcinomas by two-dimensional gel electrophoresis

J. Liu, P. Gu, G. Bergman, G. J. Kelloff, C. W. Boone, M. You, Y. Wang

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

DNA from 14 mouse lung adenocarcinomas and 7 normal lungs were examined by 2-dimensional gel electrophoresis (2-DGE) for genetic alterations. 2-DGE profiles from tumor samples were compared with those profiles from normal lung tissues through a computer-assisted color overlay system. Compared to the profiles in normal lung DNA, 6 spots were amplified and 16 spots were partially reduced in their intensity in tumors. Two spots were detectable only in tumor tissues. These altered spots indicate genetic changes in mouse lung tumor development. The identification of these genetic alterations is probably important in understanding mouse lung carcinogenesis.

Original language	English (US)
Pages (from-to)	651-658
Number of pages	8
Journal	Experimental Lung Research
Volume	26
Issue number	8
DOIs	https://doi.org/10.1080/01902140150216738
State	Published - 2000

Keywords

2-dimensional gel electrophoresis
Genetic alterations
Lung tumors
Mouse

ASJC Scopus subject areas

Molecular Biology
Pulmonary and Respiratory Medicine
Clinical Biochemistry

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10.1080/01902140150216738

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title = "Detection of genetic alterations in mouse lung adenocarcinomas by two-dimensional gel electrophoresis",

abstract = "DNA from 14 mouse lung adenocarcinomas and 7 normal lungs were examined by 2-dimensional gel electrophoresis (2-DGE) for genetic alterations. 2-DGE profiles from tumor samples were compared with those profiles from normal lung tissues through a computer-assisted color overlay system. Compared to the profiles in normal lung DNA, 6 spots were amplified and 16 spots were partially reduced in their intensity in tumors. Two spots were detectable only in tumor tissues. These altered spots indicate genetic changes in mouse lung tumor development. The identification of these genetic alterations is probably important in understanding mouse lung carcinogenesis.",

keywords = "2-dimensional gel electrophoresis, Genetic alterations, Lung tumors, Mouse",

author = "J. Liu and P. Gu and G. Bergman and Kelloff, {G. J.} and Boone, {C. W.} and M. You and Y. Wang",

note = "Funding Information: Received 20 July 2000; accepted 1 August 2000. J. L. and P. G. contributed equally to this work. This work was supported by USPS GHrants CA545. We8than5k Drs. Gary D. Stoner, Herman A. J.",

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language = "English (US)",

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T1 - Detection of genetic alterations in mouse lung adenocarcinomas by two-dimensional gel electrophoresis

AU - Liu, J.

AU - Gu, P.

AU - Bergman, G.

AU - Kelloff, G. J.

AU - Boone, C. W.

AU - You, M.

AU - Wang, Y.

N1 - Funding Information: Received 20 July 2000; accepted 1 August 2000. J. L. and P. G. contributed equally to this work. This work was supported by USPS GHrants CA545. We8than5k Drs. Gary D. Stoner, Herman A. J.

PY - 2000

Y1 - 2000

N2 - DNA from 14 mouse lung adenocarcinomas and 7 normal lungs were examined by 2-dimensional gel electrophoresis (2-DGE) for genetic alterations. 2-DGE profiles from tumor samples were compared with those profiles from normal lung tissues through a computer-assisted color overlay system. Compared to the profiles in normal lung DNA, 6 spots were amplified and 16 spots were partially reduced in their intensity in tumors. Two spots were detectable only in tumor tissues. These altered spots indicate genetic changes in mouse lung tumor development. The identification of these genetic alterations is probably important in understanding mouse lung carcinogenesis.

AB - DNA from 14 mouse lung adenocarcinomas and 7 normal lungs were examined by 2-dimensional gel electrophoresis (2-DGE) for genetic alterations. 2-DGE profiles from tumor samples were compared with those profiles from normal lung tissues through a computer-assisted color overlay system. Compared to the profiles in normal lung DNA, 6 spots were amplified and 16 spots were partially reduced in their intensity in tumors. Two spots were detectable only in tumor tissues. These altered spots indicate genetic changes in mouse lung tumor development. The identification of these genetic alterations is probably important in understanding mouse lung carcinogenesis.

KW - 2-dimensional gel electrophoresis

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Detection of genetic alterations in mouse lung adenocarcinomas by two-dimensional gel electrophoresis

Abstract

Keywords

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