Detection of a target protein (GroEl2) in Mycobacterium tuberculosis using a derivative of 1,2,4-triazolethiols

Sampa Sarkar, Sagar Swami, Sarvesh Kumar Soni, Jessica K. Holien, Arshad Khan, Arvind M. Korwar, Anjali P. Likhite, Ramesh A. Joshi, Rohini R. Joshi, Dhiman Sarkar

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Herein, we identified a potent lead compound RRA2, within a series of 54 derivatives of 1,2,4-triazolethiols (exhibit good potency as an anti-mycobacterial agents) against intracellular Mycobacterium tuberculosis (Mtb). Compound RRA2 showed significant mycobactericidal activity against active stage Mycobacterium bovis BCG and Mtb with minimum inhibitory concentration (MIC) values of 2.3 and 2.0 µg/mL, respectively. At MIC value, RRA2 compound yielded 0.82 log reduction of colony-forming unit (cfu) against non-replicating Mtb. Furthermore, RRA2 compound was selected for further target identification due to the presence of alkyne group, showing higher selectivity index (> 66.66 ± 0.22, in non-replicating stage). Using “click” chemistry, we synthesized the biotin linker-RRA2 conjugate, purified with HPLC method and confirmed the conjugation of biotin linker-RRA2 complex by HR-MS analysis. Furthermore, we successfully pulled down and identified a specific target protein GroEl2, from Mtb whole-cell extract. Furthermore, computational molecular modeling indicated RRA2 could interact with GroEl2, which explains the structure–activity relationship observed in this study. Graphical abstract: GroEL-2 identified a potent and specific target protein for RRA 2 compound in whole cell extract of Mtb H37Ra. [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)2535-2548
Number of pages14
JournalMolecular Diversity
Volume26
Issue number5
DOIs
StatePublished - Oct 2022

Keywords

  • 1,2,4-triazolethiols
  • GroEl2
  • Mycobactericidal activity
  • Mycobacterium bovis BCG
  • Mycobacterium tuberculosis

ASJC Scopus subject areas

  • Catalysis
  • Information Systems
  • Molecular Biology
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint

Dive into the research topics of 'Detection of a target protein (GroEl2) in Mycobacterium tuberculosis using a derivative of 1,2,4-triazolethiols'. Together they form a unique fingerprint.

Cite this