Detection and identification of nucleic acid engineered fluorescent labels in submicrometre fluidic channels

Samuel M. Stavis, Joshua B. Edel, Yougen Li, Kevan T. Samiee, Dan Luo, Harold G. Craighead

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

A method to detect and identify nucleic acid engineered fluorescent labels in submicrometer fluidic channels was presented. Labels with small differences in fluorophore composition were differentiated with varying degrees of accuracy and synthesized at the molecular level from dendrimer-like DNA, with the identity encoded into the number of Alexa Fluor 488and BODIPY 630/650 fluorophores conjugated with the structure. Labels with a difference of two fluorophores of each color were individually distinguishable with 80% ± 1%) accuracy. It was found that flow speed and surface interactions were important experimental parameters affecting molecular detection efficiency and throughput.

Original languageEnglish (US)
JournalNanotechnology
Volume16
Issue number7
DOIs
StatePublished - Jul 1 2005

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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