Detection and identification of nucleic acid engineered fluorescent labels in submicrometre fluidic channels

Samuel M. Stavis; Joshua B. Edel; Yougen Li; Kevan T. Samiee; Dan Luo; Harold G. Craighead

doi:10.1088/0957-4484/16/7/002

Detection and identification of nucleic acid engineered fluorescent labels in submicrometre fluidic channels

Samuel M. Stavis, Joshua B. Edel, Yougen Li, Kevan T. Samiee, Dan Luo, Harold G. Craighead

Houston Methodist

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

A method to detect and identify nucleic acid engineered fluorescent labels in submicrometer fluidic channels was presented. Labels with small differences in fluorophore composition were differentiated with varying degrees of accuracy and synthesized at the molecular level from dendrimer-like DNA, with the identity encoded into the number of Alexa Fluor 488and BODIPY 630/650 fluorophores conjugated with the structure. Labels with a difference of two fluorophores of each color were individually distinguishable with 80% ± 1%) accuracy. It was found that flow speed and surface interactions were important experimental parameters affecting molecular detection efficiency and throughput.

Original language	English (US)
Pages (from-to)	S314-S323
Journal	Nanotechnology
Volume	16
Issue number	7
DOIs	https://doi.org/10.1088/0957-4484/16/7/002
State	Published - Jul 1 2005

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Mechanics of Materials
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1088/0957-4484/16/7/002

Cite this

@article{918cdd6adca64beeb864ea3016036885,

title = "Detection and identification of nucleic acid engineered fluorescent labels in submicrometre fluidic channels",

abstract = "A method to detect and identify nucleic acid engineered fluorescent labels in submicrometer fluidic channels was presented. Labels with small differences in fluorophore composition were differentiated with varying degrees of accuracy and synthesized at the molecular level from dendrimer-like DNA, with the identity encoded into the number of Alexa Fluor 488and BODIPY 630/650 fluorophores conjugated with the structure. Labels with a difference of two fluorophores of each color were individually distinguishable with 80% ± 1%) accuracy. It was found that flow speed and surface interactions were important experimental parameters affecting molecular detection efficiency and throughput.",

author = "Stavis, {Samuel M.} and Edel, {Joshua B.} and Yougen Li and Samiee, {Kevan T.} and Dan Luo and Craighead, {Harold G.}",

year = "2005",

month = jul,

day = "1",

doi = "10.1088/0957-4484/16/7/002",

language = "English (US)",

volume = "16",

pages = "S314--S323",

journal = "Nanotechnology",

issn = "0957-4484",

publisher = "IOP Publishing Ltd.",

number = "7",

}

TY - JOUR

T1 - Detection and identification of nucleic acid engineered fluorescent labels in submicrometre fluidic channels

AU - Stavis, Samuel M.

AU - Edel, Joshua B.

AU - Li, Yougen

AU - Samiee, Kevan T.

AU - Luo, Dan

AU - Craighead, Harold G.

PY - 2005/7/1

Y1 - 2005/7/1

N2 - A method to detect and identify nucleic acid engineered fluorescent labels in submicrometer fluidic channels was presented. Labels with small differences in fluorophore composition were differentiated with varying degrees of accuracy and synthesized at the molecular level from dendrimer-like DNA, with the identity encoded into the number of Alexa Fluor 488and BODIPY 630/650 fluorophores conjugated with the structure. Labels with a difference of two fluorophores of each color were individually distinguishable with 80% ± 1%) accuracy. It was found that flow speed and surface interactions were important experimental parameters affecting molecular detection efficiency and throughput.

AB - A method to detect and identify nucleic acid engineered fluorescent labels in submicrometer fluidic channels was presented. Labels with small differences in fluorophore composition were differentiated with varying degrees of accuracy and synthesized at the molecular level from dendrimer-like DNA, with the identity encoded into the number of Alexa Fluor 488and BODIPY 630/650 fluorophores conjugated with the structure. Labels with a difference of two fluorophores of each color were individually distinguishable with 80% ± 1%) accuracy. It was found that flow speed and surface interactions were important experimental parameters affecting molecular detection efficiency and throughput.

UR - http://www.scopus.com/inward/record.url?scp=21144452102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21144452102&partnerID=8YFLogxK

U2 - 10.1088/0957-4484/16/7/002

DO - 10.1088/0957-4484/16/7/002

M3 - Article

C2 - 21727447

AN - SCOPUS:21144452102

SN - 0957-4484

VL - 16

SP - S314-S323

JO - Nanotechnology

JF - Nanotechnology

IS - 7

ER -

Detection and identification of nucleic acid engineered fluorescent labels in submicrometre fluidic channels

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this