Detecting exposure to environmental organic toxins in individual cells: towards development of a micro-fabricated device

Hoi Ying N Holman, Miqin Zhang, Regine Goth-Goldstein, Michael C. Martin, Marion Russell, Wayne R. McKinney, Mauro Ferrari, Jennie C. Hunter-Cevera

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

A new method is being developed for quickly screen for the human exposure potential to polycyclic aromatic hydrocarbons (PAHs) and organochlorines (OCs). The development involves two key elements: identifying suitable signals that represent intracellular changes that are specific to PAH and OC exposure, and constructing a device to guide the biological cell growth so that signals from individual cells are consistent and reproducible. We are completing the identification of suitable signals by using synchrotron radiation-based (SR) Fourier-transform infrared (FTIR) spectromicroscopy in the mid-infrared region (4000-400 cm-1). Distinct changes have been observed in the IR spectra after treatment of human cells in culture medium with PAHs and OCs. The potential use of this method for detecting exposure to PAHs and OCs has been tested and compared to a reverse transcription polymerase chain reaction (RT-PCR) assay that quantifies increased expression of the CYP1A1 gene in response to exposure to PAHs or OCs.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages55-62
Number of pages8
Volume3606
ISBN (Print)0819430765
StatePublished - Jan 1 1999
EventProceedings of the 1999 Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications II - San Jose, CA, USA
Duration: Jan 25 1999Jan 26 1999

Other

OtherProceedings of the 1999 Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications II
CitySan Jose, CA, USA
Period1/25/991/26/99

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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