Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor

J. Stolarov; K. Chang; A. Reiner; L. Rodgers; G. J. Hannon; M. H. Wigler; V. Mittal

doi:10.1073/pnas.221450598

Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor

J. Stolarov, K. Chang, A. Reiner, L. Rodgers, G. J. Hannon, M. H. Wigler, V. Mittal

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

We have engineered the ecdysone-inducible mammalian expression system for general retroviral delivery to cultured mammalian cells. We inducibly expressed PTEN in the glioblastoma cell line, U87MG, lacking this gene. Because nearly all cells are recruited on induction, we find both up- and down-regulated genes by cDNA microarray analysis. The changes we see are similar to those observed after treatment with LY294002, an inhibitor of phosphatidylinositol 3-OH kinase, fully consistent with the model that PTEN antagonizes phosphatidylinositol 3-OH kinase. Both treatments result in suppressed expression of the transforming growth factor (TGF)-β gene and the genes of the cholesterol biosynthesis pathway. Our results illustrate the power of using a fully inducible expression system in conjunction with cDNA microarray analysis for exploring gene function.

Original language	English (US)
Pages (from-to)	13043-13048
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	98
Issue number	23
DOIs	https://doi.org/10.1073/pnas.221450598
State	Published - Nov 6 2001

Keywords

DNA microarray
Glioblastoma
Inducible expression

ASJC Scopus subject areas

Genetics
General

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10.1073/pnas.221450598

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Stolarov, J., Chang, K., Reiner, A., Rodgers, L., Hannon, G. J., Wigler, M. H., & Mittal, V. (2001). Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor. Proceedings of the National Academy of Sciences of the United States of America, 98(23), 13043-13048. https://doi.org/10.1073/pnas.221450598

Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor. / Stolarov, J.; Chang, K.; Reiner, A. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 23, 06.11.2001, p. 13043-13048.

Research output: Contribution to journal › Article › peer-review

Stolarov, J, Chang, K, Reiner, A, Rodgers, L, Hannon, GJ, Wigler, MH & Mittal, V 2001, 'Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor', Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 23, pp. 13043-13048. https://doi.org/10.1073/pnas.221450598

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Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor

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