Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor

J. Stolarov, K. Chang, A. Reiner, L. Rodgers, G. J. Hannon, M. H. Wigler, V. Mittal

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

We have engineered the ecdysone-inducible mammalian expression system for general retroviral delivery to cultured mammalian cells. We inducibly expressed PTEN in the glioblastoma cell line, U87MG, lacking this gene. Because nearly all cells are recruited on induction, we find both up- and down-regulated genes by cDNA microarray analysis. The changes we see are similar to those observed after treatment with LY294002, an inhibitor of phosphatidylinositol 3-OH kinase, fully consistent with the model that PTEN antagonizes phosphatidylinositol 3-OH kinase. Both treatments result in suppressed expression of the transforming growth factor (TGF)-β gene and the genes of the cholesterol biosynthesis pathway. Our results illustrate the power of using a fully inducible expression system in conjunction with cDNA microarray analysis for exploring gene function.

Original languageEnglish (US)
Pages (from-to)13043-13048
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number23
DOIs
StatePublished - Nov 6 2001

Keywords

  • DNA microarray
  • Glioblastoma
  • Inducible expression

ASJC Scopus subject areas

  • Genetics
  • General

Fingerprint Dive into the research topics of 'Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor'. Together they form a unique fingerprint.

Cite this