Design and characterization of a galactose biosensor using a novel polypyrrole-hydrogel composite membrane

Sean I. Brahim, Dow Maharajh, Dyer Narinesingh, Anthony Guiseppi-Elie

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

A rapid, two-step method for constructing galactose biosensors by entrapment of galactose oxidase within a polymeric composite has been developed. The composite material is formed as an interpenetrating network of polypyrrole grown within a UV cross-linked poly(2-hydroxyethyl methacrylate) [p(HEMA)] hydrogel. The optimized galactose biosensor exhibited a linear response range from 5.0 × 10-5 to 1.0 × 10-2 M and detection limit of 25 μM toward galactose. The response time of the biosensor was 70s. The analytical recovery of galactose in serum samples ranged from 97 to 105% with mean coefficients of variation of 3.8% (within-day analyses) and 4.4% (day-to-day analyses). The biosensor was effective in screening up to twice the physiological levels of ascorbate, urate and acetaminophen interferents and retained 70% of initial enzyme activity after 9 months when stored desiccated in the absence of buffer at 4°C.

Original languageEnglish (US)
Pages (from-to)797-812
Number of pages16
JournalAnalytical Letters
Volume35
Issue number5
DOIs
StatePublished - 2002

Keywords

  • Galactose biosensor
  • Galactose oxidase
  • Hydrogel composite
  • Polypyrrole

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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