Design and characterization of a galactose biosensor using a novel polypyrrole-hydrogel composite membrane

Sean I. Brahim; Dow Maharajh; Dyer Narinesingh; Anthony Guiseppi-Elie

doi:10.1081/AL-120004070

Design and characterization of a galactose biosensor using a novel polypyrrole-hydrogel composite membrane

Sean I. Brahim, Dow Maharajh, Dyer Narinesingh, Anthony Guiseppi-Elie

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

A rapid, two-step method for constructing galactose biosensors by entrapment of galactose oxidase within a polymeric composite has been developed. The composite material is formed as an interpenetrating network of polypyrrole grown within a UV cross-linked poly(2-hydroxyethyl methacrylate) [p(HEMA)] hydrogel. The optimized galactose biosensor exhibited a linear response range from 5.0 × 10^-5 to 1.0 × 10^-2 M and detection limit of 25 μM toward galactose. The response time of the biosensor was 70s. The analytical recovery of galactose in serum samples ranged from 97 to 105% with mean coefficients of variation of 3.8% (within-day analyses) and 4.4% (day-to-day analyses). The biosensor was effective in screening up to twice the physiological levels of ascorbate, urate and acetaminophen interferents and retained 70% of initial enzyme activity after 9 months when stored desiccated in the absence of buffer at 4°C.

Original language	English (US)
Pages (from-to)	797-812
Number of pages	16
Journal	Analytical Letters
Volume	35
Issue number	5
DOIs	https://doi.org/10.1081/AL-120004070
State	Published - 2002

Keywords

Galactose biosensor
Galactose oxidase
Hydrogel composite
Polypyrrole

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

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10.1081/AL-120004070

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title = "Design and characterization of a galactose biosensor using a novel polypyrrole-hydrogel composite membrane",

abstract = "A rapid, two-step method for constructing galactose biosensors by entrapment of galactose oxidase within a polymeric composite has been developed. The composite material is formed as an interpenetrating network of polypyrrole grown within a UV cross-linked poly(2-hydroxyethyl methacrylate) [p(HEMA)] hydrogel. The optimized galactose biosensor exhibited a linear response range from 5.0 × 10-5 to 1.0 × 10-2 M and detection limit of 25 μM toward galactose. The response time of the biosensor was 70s. The analytical recovery of galactose in serum samples ranged from 97 to 105% with mean coefficients of variation of 3.8% (within-day analyses) and 4.4% (day-to-day analyses). The biosensor was effective in screening up to twice the physiological levels of ascorbate, urate and acetaminophen interferents and retained 70% of initial enzyme activity after 9 months when stored desiccated in the absence of buffer at 4°C.",

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author = "Brahim, {Sean I.} and Dow Maharajh and Dyer Narinesingh and Anthony Guiseppi-Elie",

note = "Funding Information: The authors gratefully acknowledge the VCU Center for Bioelectronics, Biosensors and Biochips (C3B), the Virginia Center for Innovative Technology (CIT BIO-99-010) and ABTECH Scientific, Inc. for financial support. SB thanks Lebert Grierson for useful discussions and the University of the West Indies for a post-graduate scholarship. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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AU - Narinesingh, Dyer

AU - Guiseppi-Elie, Anthony

N1 - Funding Information: The authors gratefully acknowledge the VCU Center for Bioelectronics, Biosensors and Biochips (C3B), the Virginia Center for Innovative Technology (CIT BIO-99-010) and ABTECH Scientific, Inc. for financial support. SB thanks Lebert Grierson for useful discussions and the University of the West Indies for a post-graduate scholarship. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2002

Y1 - 2002

N2 - A rapid, two-step method for constructing galactose biosensors by entrapment of galactose oxidase within a polymeric composite has been developed. The composite material is formed as an interpenetrating network of polypyrrole grown within a UV cross-linked poly(2-hydroxyethyl methacrylate) [p(HEMA)] hydrogel. The optimized galactose biosensor exhibited a linear response range from 5.0 × 10-5 to 1.0 × 10-2 M and detection limit of 25 μM toward galactose. The response time of the biosensor was 70s. The analytical recovery of galactose in serum samples ranged from 97 to 105% with mean coefficients of variation of 3.8% (within-day analyses) and 4.4% (day-to-day analyses). The biosensor was effective in screening up to twice the physiological levels of ascorbate, urate and acetaminophen interferents and retained 70% of initial enzyme activity after 9 months when stored desiccated in the absence of buffer at 4°C.

AB - A rapid, two-step method for constructing galactose biosensors by entrapment of galactose oxidase within a polymeric composite has been developed. The composite material is formed as an interpenetrating network of polypyrrole grown within a UV cross-linked poly(2-hydroxyethyl methacrylate) [p(HEMA)] hydrogel. The optimized galactose biosensor exhibited a linear response range from 5.0 × 10-5 to 1.0 × 10-2 M and detection limit of 25 μM toward galactose. The response time of the biosensor was 70s. The analytical recovery of galactose in serum samples ranged from 97 to 105% with mean coefficients of variation of 3.8% (within-day analyses) and 4.4% (day-to-day analyses). The biosensor was effective in screening up to twice the physiological levels of ascorbate, urate and acetaminophen interferents and retained 70% of initial enzyme activity after 9 months when stored desiccated in the absence of buffer at 4°C.

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Design and characterization of a galactose biosensor using a novel polypyrrole-hydrogel composite membrane

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