(-)-Deprenyl protection of 1-methyl-4 phenylpyridium ion (MPP+)-induced apoptosis independent of MAO-B inhibition

Weidong Le, Joseph Jankovic, Wenjie Xie, Rong Kong, Stanley H. Appel

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Selective loss of central dopaminergic neurons in vitro and in vivo can be initiated by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) through its metabolite phenylpyridium ion (MPP+). Such MPTP-mediated cytotoxicity can be blocked by (-)-Deprenyl, a monoamine oxidase (MAO)-B inhibitor, but the exact mechanisms of MPP+-induced cytotoxicity and (-)-Deprenyl's protection against such neurotoxicity are not fully understood. Using a hybrid clone MES 23.5, a dopaminergic cell line that does not contain MAO-B, we document that MPP+ induces apoptotic cell death. Application of (-)-Deprenyl at concentrations of 0.1-10 μM significantly reduces MPP+-induced apoptosis in MES 23.5 cells; (-)-Deprenyl at higher concentrations (> 100 μM) that completely inhibit MAO-B activity, however, induces apoptosis. Pretreatment with N-(2-aminoethyl)-p-chlorobenzamide (Ro 16-6491), a selective MAO-B inhibitor, does not protect MES 23.5 cells against MPP+-induced cell death. These results suggest that the protective action of (-)-Deprenyl against MPP+ neurotoxicity in dopaminergic cell line may be independent of the inhibition of MAO-B.

Original languageEnglish (US)
Pages (from-to)197-200
Number of pages4
JournalNeuroscience Letters
Volume224
Issue number3
DOIs
StatePublished - Apr 10 1997

Keywords

  • (-)-Deprenyl
  • 1-Methyl-4-phenylpyridinium ion
  • Apoptosis
  • Monoamine oxidase-B
  • Parkinson's disease

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of '(-)-Deprenyl protection of 1-methyl-4 phenylpyridium ion (MPP<sup>+</sup>)-induced apoptosis independent of MAO-B inhibition'. Together they form a unique fingerprint.

Cite this