Denaturation of DNA after X-irradiation in vitro

Ranjit Das Gupta; Sankar Mitra

doi:10.1016/0006-291X(70)90972-1

Denaturation of DNA after X-irradiation in vitro

Ranjit Das Gupta, Sankar Mitra

Academic Institute

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

X-irradiation of E. coli DNA caused partial strand-separation as judged by susceptibility of the DNA to nuclease specific for single-stranded DNA, namely an endonuclease from Neurospora crassa and exonuclease I of E. coli. The elution profile of X-irradiated DNA from hydroxyapatite column showed that single-stranded fragments were produced. These fragments had a sedimentation coefficient of 3-4s corresponding to a length of about 250 nucleotides.

Original language	English (US)
Pages (from-to)	793-799
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	40
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(70)90972-1
State	Published - Aug 24 1970

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(70)90972-1

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title = "Denaturation of DNA after X-irradiation in vitro",

abstract = "X-irradiation of E. coli DNA caused partial strand-separation as judged by susceptibility of the DNA to nuclease specific for single-stranded DNA, namely an endonuclease from Neurospora crassa and exonuclease I of E. coli. The elution profile of X-irradiated DNA from hydroxyapatite column showed that single-stranded fragments were produced. These fragments had a sedimentation coefficient of 3-4s corresponding to a length of about 250 nucleotides.",

author = "\{Das Gupta\}, Ranjit and Sankar Mitra",

note = "Funding Information: The authors wish to thank the Council of Scientific and Industrial Research, India for a research grant. Dr. S.B. Bhattacharjee of Saha Institute of Nuclear Physics, Calcutta kindly made available their X-ray generator. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1970",

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doi = "10.1016/0006-291X(70)90972-1",

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AU - Das Gupta, Ranjit

AU - Mitra, Sankar

N1 - Funding Information: The authors wish to thank the Council of Scientific and Industrial Research, India for a research grant. Dr. S.B. Bhattacharjee of Saha Institute of Nuclear Physics, Calcutta kindly made available their X-ray generator. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1970/8/24

Y1 - 1970/8/24

N2 - X-irradiation of E. coli DNA caused partial strand-separation as judged by susceptibility of the DNA to nuclease specific for single-stranded DNA, namely an endonuclease from Neurospora crassa and exonuclease I of E. coli. The elution profile of X-irradiated DNA from hydroxyapatite column showed that single-stranded fragments were produced. These fragments had a sedimentation coefficient of 3-4s corresponding to a length of about 250 nucleotides.

AB - X-irradiation of E. coli DNA caused partial strand-separation as judged by susceptibility of the DNA to nuclease specific for single-stranded DNA, namely an endonuclease from Neurospora crassa and exonuclease I of E. coli. The elution profile of X-irradiated DNA from hydroxyapatite column showed that single-stranded fragments were produced. These fragments had a sedimentation coefficient of 3-4s corresponding to a length of about 250 nucleotides.

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JO - Biochemical and Biophysical Research Communications

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IS - 4

ER -

Denaturation of DNA after X-irradiation in vitro

Abstract

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