Denaturation of DNA after X-irradiation in vitro

Ranjit Das Gupta, Sankar Mitra

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

X-irradiation of E. coli DNA caused partial strand-separation as judged by susceptibility of the DNA to nuclease specific for single-stranded DNA, namely an endonuclease from Neurospora crassa and exonuclease I of E. coli. The elution profile of X-irradiated DNA from hydroxyapatite column showed that single-stranded fragments were produced. These fragments had a sedimentation coefficient of 3-4s corresponding to a length of about 250 nucleotides.

Original languageEnglish (US)
Pages (from-to)793-799
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume40
Issue number4
DOIs
StatePublished - Aug 24 1970

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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