Demethylation of O6-methylguanine in a synthetic DNA polymer by an inducible activity in Escherichiacoli

R. S. Foote; S. Mitra; B. C. Pal

doi:10.1016/0006-291X(80)90314-9

Demethylation of O⁶-methylguanine in a synthetic DNA polymer by an inducible activity in Escherichiacoli

R. S. Foote, S. Mitra, B. C. Pal

Research output: Contribution to journal › Article › peer-review

138 Scopus citations

Abstract

O⁶-Methyl[8-³H]deoxyguanosine in a synthetic DNA polymer, poly(dC, dG, m⁶dG), is demethylated by cell-free extracts of EscherichiacoliB/r adapted by exposure to N-methyl-N′-nitro-N-nitrosoguanidine, as shown by the appearance of ³H-labeled deoxyguanosine in hydrolysates of the recovered DNA. The demethylating activity could not be detected in extracts of nonadapted E. coli. These results provide direct evidence that a previously described inducible repair activity in E. coli acts by demethylating O⁶-methylguanine at the DNA level.

Original language	English (US)
Pages (from-to)	654-659
Number of pages	6
Journal	Topics in Catalysis
Volume	97
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(80)90314-9
State	Published - 1980

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Biophysics
Molecular Biology

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title = "Demethylation of O6-methylguanine in a synthetic DNA polymer by an inducible activity in Escherichiacoli",

abstract = "O6-Methyl[8-3H]deoxyguanosine in a synthetic DNA polymer, poly(dC, dG, m6dG), is demethylated by cell-free extracts of EscherichiacoliB/r adapted by exposure to N-methyl-N′-nitro-N-nitrosoguanidine, as shown by the appearance of 3H-labeled deoxyguanosine in hydrolysates of the recovered DNA. The demethylating activity could not be detected in extracts of nonadapted E. coli. These results provide direct evidence that a previously described inducible repair activity in E. coli acts by demethylating O6-methylguanine at the DNA level.",

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year = "1980",

doi = "10.1016/0006-291X(80)90314-9",

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AU - Foote, R. S.

AU - Mitra, S.

AU - Pal, B. C.

PY - 1980

Y1 - 1980

N2 - O6-Methyl[8-3H]deoxyguanosine in a synthetic DNA polymer, poly(dC, dG, m6dG), is demethylated by cell-free extracts of EscherichiacoliB/r adapted by exposure to N-methyl-N′-nitro-N-nitrosoguanidine, as shown by the appearance of 3H-labeled deoxyguanosine in hydrolysates of the recovered DNA. The demethylating activity could not be detected in extracts of nonadapted E. coli. These results provide direct evidence that a previously described inducible repair activity in E. coli acts by demethylating O6-methylguanine at the DNA level.

AB - O6-Methyl[8-3H]deoxyguanosine in a synthetic DNA polymer, poly(dC, dG, m6dG), is demethylated by cell-free extracts of EscherichiacoliB/r adapted by exposure to N-methyl-N′-nitro-N-nitrosoguanidine, as shown by the appearance of 3H-labeled deoxyguanosine in hydrolysates of the recovered DNA. The demethylating activity could not be detected in extracts of nonadapted E. coli. These results provide direct evidence that a previously described inducible repair activity in E. coli acts by demethylating O6-methylguanine at the DNA level.

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ER -

Demethylation of O⁶-methylguanine in a synthetic DNA polymer by an inducible activity in Escherichiacoli

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