Demethylation of O6-methylguanine in a synthetic DNA polymer by an inducible activity in Escherichiacoli

R. S. Foote, Sankar Mitra, B. C. Pal

Research output: Contribution to journalArticle

133 Scopus citations

Abstract

O6-Methyl[8-3H]deoxyguanosine in a synthetic DNA polymer, poly(dC, dG, m6dG), is demethylated by cell-free extracts of EscherichiacoliB/r adapted by exposure to N-methyl-N′-nitro-N-nitrosoguanidine, as shown by the appearance of 3H-labeled deoxyguanosine in hydrolysates of the recovered DNA. The demethylating activity could not be detected in extracts of nonadapted E. coli. These results provide direct evidence that a previously described inducible repair activity in E. coli acts by demethylating O6-methylguanine at the DNA level.

Original languageEnglish (US)
Pages (from-to)654-659
Number of pages6
JournalTopics in Catalysis
Volume97
Issue number2
DOIs
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Biophysics
  • Molecular Biology

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