Deletion of CD39 on natural killer cells attenuates hepatic ischemia/reperfusion injury in mice

Guido Beldi, Yara Banz, Alexander Kroemer, Xiaofeng Sun, Yan Wu, Nadine Graubardt, Alyssa Rellstab, Martina Nowak, Keiichi Enjyoji, Xian Li, Wolfgang G. Junger, Daniel Candinas, Simon C. Robson

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

Natural killer (NK) cells play crucial roles in innate immunity and express CD39 (Ectonucleoside triphosphate diphosphohydrolase 1 [E-NTPD1]), a rate-limiting ectonucleotidase in the phosphohydrolysis of extracellular nucleotides to adenosine. We have studied the effects of CD39 gene deletion on NK cells in dictating outcomes after partial hepatic ischemia/reperfusion injury (IRI). We show in mice that gene deletion of CD39 is associated with marked decreases in phosphohydrolysis of adenosine triphosphate (ATP) and adenosine diphosphate to adenosine monophosphate on NK cells, thereby modulating the type-2 purinergic (P2) receptors demonstrated on these cells. We note that CD39-null mice are protected from acute vascular injury after single-lobe warm IRI, and, relative to control wild-type mice, display significantly less elevation of aminotransferases with less pronounced histopathological changes associated with IRI. Selective adoptive transfers of immune cells into Rag2/common gamma null mice (deficient in T cells, B cells, and NK/NKT cells) suggest that it is CD39 deletion on NK cells that provides end-organ protection, which is comparable to that seen in the absence of interferon gamma. Indeed, NK effector mechanisms such as interferon gamma secretion are inhibited by P2 receptor activation in vitro. Specifically, ATPγS (a nonhydrolyzable ATP analog) inhibits secretion of interferon gamma by NK cells in response to interleukin-12 and interleukin-18, providing a mechanistic link between CD39 deletion and altered cytokine secretion. Conclusion: We propose that CD39 deficiency and changes in P2 receptor activation abrogate secretion of interferon gamma by NK cells in response to inflammatory mediators, thereby limiting tissue damage mediated by these innate immune cells during IRI.

Original languageEnglish (US)
Pages (from-to)1702-1711
Number of pages10
JournalHepatology
Volume51
Issue number5
DOIs
StatePublished - May 2010

ASJC Scopus subject areas

  • Hepatology

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