TY - JOUR
T1 - Deletion of atoR from Streptococcus pyogenes Results in hypervirulence in a mouse model of sepsis and is LuxS independent
AU - Sitkiewicz, Izabela
AU - Musser, James M.
N1 - Funding Information:
This work was supported by N N303 822240 grant to I.S. and funds from the Fondren Foundation to J.M.M.
PY - 2017
Y1 - 2017
N2 - Group A Streptococcus (GAS) is a Gram-positive human pathogen that causes a variety of diseases ranging from pharyngitis to life-threatening streptococcal toxic shock syndrome. Recently, several global gene expression analyses have yielded extensive new information regarding the regulation of genes encoding known and putative virulence factors in GAS. A microarray analysis found that transcription of the GAS gene M5005-Spy-1343 was significantly increased in response to interaction with human polymorphonuclear leukocytes. M5005-Spy-1343 is predicted to encode a member of the LysR family of transcriptional regulators and is located upstream of a putative operon containing six genes. Five of these genes have sequence similarity to genes involved in short-chain fatty acid metabolism, whereas the sixth gene (luxS) is found in many bacterial species and is involved in quorum sensing. Unexpectedly, inactivation of the M5005-Spy-1343 gene resulted in hypervirulence in an intraperitoneal mouse model of infection. Increased virulence was not due to changes in luxS gene expression. We postulate that short-chain fatty acid metabolism is involved in GAS pathogenesis.
AB - Group A Streptococcus (GAS) is a Gram-positive human pathogen that causes a variety of diseases ranging from pharyngitis to life-threatening streptococcal toxic shock syndrome. Recently, several global gene expression analyses have yielded extensive new information regarding the regulation of genes encoding known and putative virulence factors in GAS. A microarray analysis found that transcription of the GAS gene M5005-Spy-1343 was significantly increased in response to interaction with human polymorphonuclear leukocytes. M5005-Spy-1343 is predicted to encode a member of the LysR family of transcriptional regulators and is located upstream of a putative operon containing six genes. Five of these genes have sequence similarity to genes involved in short-chain fatty acid metabolism, whereas the sixth gene (luxS) is found in many bacterial species and is involved in quorum sensing. Unexpectedly, inactivation of the M5005-Spy-1343 gene resulted in hypervirulence in an intraperitoneal mouse model of infection. Increased virulence was not due to changes in luxS gene expression. We postulate that short-chain fatty acid metabolism is involved in GAS pathogenesis.
KW - Ato
KW - Host-pathogen interactions
KW - Short chain fatty acid synthesis
KW - Streptococcus pyogenes
KW - Virulence factors
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U2 - 10.5604/17331331.1234989
DO - 10.5604/17331331.1234989
M3 - Article
C2 - 29359701
AN - SCOPUS:85028935321
SN - 1733-1331
VL - 66
SP - 17
EP - 24
JO - Polish Journal of Microbiology
JF - Polish Journal of Microbiology
IS - 1
ER -