D2/D3 receptor agonist ropinirole protects dopaminergic cell line against rotenone-induced apoptosis through inhibition of caspase- and JNK-dependent pathways

Sheng Chen; Xiaojie Zhang; Dehua Yang; Yunlan Du; Liang Li; Xuping Li; Ming Ming; Weidong Le

doi:10.1016/j.febslet.2008.01.028

D2/D3 receptor agonist ropinirole protects dopaminergic cell line against rotenone-induced apoptosis through inhibition of caspase- and JNK-dependent pathways

Sheng Chen, Xiaojie Zhang, Dehua Yang, Yunlan Du, Liang Li, Xuping Li, Ming Ming, Weidong Le

Research output: Contribution to journal › Article

42 Scopus citations

Abstract

Ropinirole, a D2/D3 receptor agonist has been reported to have neuroprotective effects. We showed that ropinirole can prevent rotenone-induced apoptosis in dopaminergic cell line SH-SY5Y through D3 receptor. We found that ropinirole can block the rotenone-induced phosphorylation of JNK, P38 and p-c-Jun, but promote the phosphorylation of ERK1/2. Furthermore, we demonstrated that ropinirole can reduce the rotenone-induced cleavages of caspase 9, caspase 3 and PARP and elevate the expression of anti-apoptotic proteins of p-Akt and bcl-2. These results provide a basis for neuroprotection by this drug for the treatment of Parkinson disease.

Original language	English (US)
Pages (from-to)	603-610
Number of pages	8
Journal	FEBS Letters
Volume	582
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2008.01.028
State	Published - Mar 5 2008

Keywords

Apoptosis
MAP-kinase
Parkinson disease
Ropinirole
Rotenone
SH-SY5Y cell line

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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D2/D3 receptor agonist ropinirole protects dopaminergic cell line against rotenone-induced apoptosis through inhibition of caspase- and JNK-dependent pathways. / Chen, Sheng; Zhang, Xiaojie; Yang, Dehua; Du, Yunlan; Li, Liang; Li, Xuping; Ming, Ming; Le, Weidong.

In: FEBS Letters, Vol. 582, No. 5, 05.03.2008, p. 603-610.

Research output: Contribution to journal › Article

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AU - Du, Yunlan

AU - Li, Liang

AU - Li, Xuping

AU - Ming, Ming

AU - Le, Weidong

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AB - Ropinirole, a D2/D3 receptor agonist has been reported to have neuroprotective effects. We showed that ropinirole can prevent rotenone-induced apoptosis in dopaminergic cell line SH-SY5Y through D3 receptor. We found that ropinirole can block the rotenone-induced phosphorylation of JNK, P38 and p-c-Jun, but promote the phosphorylation of ERK1/2. Furthermore, we demonstrated that ropinirole can reduce the rotenone-induced cleavages of caspase 9, caspase 3 and PARP and elevate the expression of anti-apoptotic proteins of p-Akt and bcl-2. These results provide a basis for neuroprotection by this drug for the treatment of Parkinson disease.

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