D2/D3 receptor agonist ropinirole protects dopaminergic cell line against rotenone-induced apoptosis through inhibition of caspase- and JNK-dependent pathways

Sheng Chen; Xiaojie Zhang; Dehua Yang; Yunlan Du; Liang Li; Xuping Li; Ming Ming; Weidong Le

doi:10.1016/j.febslet.2008.01.028

D2/D3 receptor agonist ropinirole protects dopaminergic cell line against rotenone-induced apoptosis through inhibition of caspase- and JNK-dependent pathways

Sheng Chen, Xiaojie Zhang, Dehua Yang, Yunlan Du, Liang Li, Xuping Li, Ming Ming, Weidong Le

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Ropinirole, a D2/D3 receptor agonist has been reported to have neuroprotective effects. We showed that ropinirole can prevent rotenone-induced apoptosis in dopaminergic cell line SH-SY5Y through D3 receptor. We found that ropinirole can block the rotenone-induced phosphorylation of JNK, P38 and p-c-Jun, but promote the phosphorylation of ERK1/2. Furthermore, we demonstrated that ropinirole can reduce the rotenone-induced cleavages of caspase 9, caspase 3 and PARP and elevate the expression of anti-apoptotic proteins of p-Akt and bcl-2. These results provide a basis for neuroprotection by this drug for the treatment of Parkinson disease.

Original language	English (US)
Pages (from-to)	603-610
Number of pages	8
Journal	FEBS Letters
Volume	582
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2008.01.028
State	Published - Mar 5 2008

Keywords

Apoptosis
MAP-kinase
Parkinson disease
Ropinirole
Rotenone
SH-SY5Y cell line

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/j.febslet.2008.01.028

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title = "D2/D3 receptor agonist ropinirole protects dopaminergic cell line against rotenone-induced apoptosis through inhibition of caspase- and JNK-dependent pathways",

abstract = "Ropinirole, a D2/D3 receptor agonist has been reported to have neuroprotective effects. We showed that ropinirole can prevent rotenone-induced apoptosis in dopaminergic cell line SH-SY5Y through D3 receptor. We found that ropinirole can block the rotenone-induced phosphorylation of JNK, P38 and p-c-Jun, but promote the phosphorylation of ERK1/2. Furthermore, we demonstrated that ropinirole can reduce the rotenone-induced cleavages of caspase 9, caspase 3 and PARP and elevate the expression of anti-apoptotic proteins of p-Akt and bcl-2. These results provide a basis for neuroprotection by this drug for the treatment of Parkinson disease.",

keywords = "Apoptosis, MAP-kinase, Parkinson disease, Ropinirole, Rotenone, SH-SY5Y cell line",

author = "Sheng Chen and Xiaojie Zhang and Dehua Yang and Yunlan Du and Liang Li and Xuping Li and Ming Ming and Weidong Le",

note = "Funding Information: This work was supported by National Science Foundation of China (No. 30370491). We appreciated Prof. John A. Dani (Division of Neuroscience, Baylor College of Medicine) for critical review of this paper.",

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doi = "10.1016/j.febslet.2008.01.028",

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T1 - D2/D3 receptor agonist ropinirole protects dopaminergic cell line against rotenone-induced apoptosis through inhibition of caspase- and JNK-dependent pathways

AU - Chen, Sheng

AU - Zhang, Xiaojie

AU - Yang, Dehua

AU - Du, Yunlan

AU - Li, Liang

AU - Li, Xuping

AU - Ming, Ming

AU - Le, Weidong

N1 - Funding Information: This work was supported by National Science Foundation of China (No. 30370491). We appreciated Prof. John A. Dani (Division of Neuroscience, Baylor College of Medicine) for critical review of this paper.

PY - 2008/3/5

Y1 - 2008/3/5

N2 - Ropinirole, a D2/D3 receptor agonist has been reported to have neuroprotective effects. We showed that ropinirole can prevent rotenone-induced apoptosis in dopaminergic cell line SH-SY5Y through D3 receptor. We found that ropinirole can block the rotenone-induced phosphorylation of JNK, P38 and p-c-Jun, but promote the phosphorylation of ERK1/2. Furthermore, we demonstrated that ropinirole can reduce the rotenone-induced cleavages of caspase 9, caspase 3 and PARP and elevate the expression of anti-apoptotic proteins of p-Akt and bcl-2. These results provide a basis for neuroprotection by this drug for the treatment of Parkinson disease.

AB - Ropinirole, a D2/D3 receptor agonist has been reported to have neuroprotective effects. We showed that ropinirole can prevent rotenone-induced apoptosis in dopaminergic cell line SH-SY5Y through D3 receptor. We found that ropinirole can block the rotenone-induced phosphorylation of JNK, P38 and p-c-Jun, but promote the phosphorylation of ERK1/2. Furthermore, we demonstrated that ropinirole can reduce the rotenone-induced cleavages of caspase 9, caspase 3 and PARP and elevate the expression of anti-apoptotic proteins of p-Akt and bcl-2. These results provide a basis for neuroprotection by this drug for the treatment of Parkinson disease.

KW - Apoptosis

KW - MAP-kinase

KW - Parkinson disease

KW - Ropinirole

KW - Rotenone

KW - SH-SY5Y cell line

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D2/D3 receptor agonist ropinirole protects dopaminergic cell line against rotenone-induced apoptosis through inhibition of caspase- and JNK-dependent pathways

Abstract

Keywords

ASJC Scopus subject areas

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