Cytoskeleton and Cytoskeleton-Bound RNA Visualization in Frog and Insect Oocytes

Malgorzata Kloc, Szczepan Bilinski, Jacek Z Kubiak

Research output: Contribution to journalArticle

Abstract

The majority of oocyte functions involves and depends on the cytoskeletal elements, which include microtubules and actin and cytokeratin filaments. Various structures and molecules are temporarily or permanently bound to the cytoskeletal elements and their functions rely on cytoskeleton integrity and its timely assembly. Thus the accurate visualization of cytoskeleton is often crucial for studies and analyses of oocyte structure and functions. Here we describe several reliable methods for microtubule and/or microfilaments preservation and visualization in Xenopus oocyte extracts, and in situ in live and fixed insect and frog (Xenopus) oocytes. In addition, we describe visualization of cytoskeleton-bound RNAs using molecular beacons in live Xenopus oocytes.

Original languageEnglish (US)
Pages (from-to)179-90
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume1457
DOIs
StatePublished - Aug 25 2016

Keywords

  • Journal Article

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