Cytometric biochips with optically active surfaces for spatial engineering of fluorescence excitation

Huw D. Summers, Rachel J. Errington, Paul Smith, Daniel R. Matthews, James F. Leary

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The development of surface-active biochips for control of fluorescence within microscopy platforms is described. These use surface-plasmon control to provide selective excitation of fluorescently labeled, live cell populations. These chips effectively combine a number of commonly used techniques such as SPR, TIRF and epi-fluorescence within a single device and have the potential to provide sub-cellular discrimination of excitation in 3-D. Thus within a single field of view we can selectively excite membrane versus cytoplasm and localise the excitation within the lateral plane to an area of a few square microns.

Original languageEnglish (US)
Title of host publicationMicrofluidics, BioMEMS, and Medical Microsystems VI
PublisherSPIE
ISBN (Print)9780819470614
DOIs
StatePublished - 2008
EventMicrofluidics, BioMEMS, and Medical Microsystems VI - San Jose, CA, United States
Duration: Jan 21 2008Jan 22 2008

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6886
ISSN (Print)0277-786X

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems VI
CountryUnited States
CitySan Jose, CA
Period1/21/081/22/08

Keywords

  • Fluorescence microscopy
  • Optical biochip
  • Optical cytometry
  • Surface plasmon

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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