TY - JOUR
T1 - Cytokine production by peripheral blood mononuclear cells in human coccidioidomycosis
AU - Corry, David B.
AU - Ampel, Neil M.
AU - Christian, Lara
AU - Locksley, Richard M.
AU - Galgiani, John N.
N1 - Funding Information:
Received 16 January 1996; revised 10 April 1996. Informed consent was obtained in accordance with guidelines of the Human Subjects Committee, University of Arizona. Grant support: American Heart Association (D.B.C.), Arizona Disease Control Research Commission (9502, N.M.A.), NIH (AI-30663), Molecular Parasitology Scholar Award from Burroughs Wellcome Fund (R.M.L.), and Department of Veterans Affairs (J.N.G.). Reprints or correspondence: Dr. Neil M. Ampel, Medical Service (III), VAMC, 3601 S. Sixth Ave., Tucson, AZ 85723.
PY - 1996
Y1 - 1996
N2 - The production and mRNA expression of the cytokines interferon-γ (IFN- γ) and interleukin (IL)-4, -10, and -12 by peripheral blood mononuclear cells (PBMC) after incubation with the coccidioidal antigen toluene spherule lysate (TSL) from various subjects were measured. The IFN-γ concentration in PBMC supernatants incubated for 72 h from 8 subjects with disseminated coccidioidomycosis was significantly less than that from 7 healthy, coccidioidal-immune subjects (P = .015). No differences were seen among the subject groups in the concentrations of IL-4, -10, or -12. By use of competitive polymerase chain reaction, PBMC from subjects with disseminated coccidioidomycosis also expressed less mRNA for IFN-γ and IL-12 than did cells from healthy, immune subjects. These data suggest that patients with disseminated coccidioidomycosis have a diminished T helper lymphocyte type 1 response.
AB - The production and mRNA expression of the cytokines interferon-γ (IFN- γ) and interleukin (IL)-4, -10, and -12 by peripheral blood mononuclear cells (PBMC) after incubation with the coccidioidal antigen toluene spherule lysate (TSL) from various subjects were measured. The IFN-γ concentration in PBMC supernatants incubated for 72 h from 8 subjects with disseminated coccidioidomycosis was significantly less than that from 7 healthy, coccidioidal-immune subjects (P = .015). No differences were seen among the subject groups in the concentrations of IL-4, -10, or -12. By use of competitive polymerase chain reaction, PBMC from subjects with disseminated coccidioidomycosis also expressed less mRNA for IFN-γ and IL-12 than did cells from healthy, immune subjects. These data suggest that patients with disseminated coccidioidomycosis have a diminished T helper lymphocyte type 1 response.
UR - https://www.scopus.com/pages/publications/0029954431
UR - https://www.scopus.com/inward/citedby.url?scp=0029954431&partnerID=8YFLogxK
U2 - 10.1093/infdis/174.2.440
DO - 10.1093/infdis/174.2.440
M3 - Article
C2 - 8699085
AN - SCOPUS:0029954431
SN - 0022-1899
VL - 174
SP - 440
EP - 443
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -