Cytochrome P4502D6 (CYP2D6) Bioelectrode for Fluoxetine

E. I. Iwuoha, A. Wilson, M. Howel, N. G.R. Mathebe, K. Montane-Jaime, D. Narinesingh, A. Guiseppi-Elie

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A bioelectrode system consisting of polyaniline (PAn)-doped glassy carbon electrode (GCE) and cytochrome P4502D6 (CYP2D6) enzyme solution was used for the amperometric study of in vitro fluoxetine biotransformation. The PAn film was potentiostatically grown at +700 mV vs. Ag/AgCl (20°C) on a 0.071 cm2 GCE and used for cyclic voltammetric (CV) measurements in phosphate buffer solution (0.1 M, pH 7.5, 0.1 M KCl) of the enzyme. The response of the CYP2D6 bioelectrode to fluoxetine was consistent with uncompetitive substrate inhibition kinetics. Apparent Michaelis-Menten constant K′m of the CYP2D6 electrode was 3.7 μmol/L, which is within the intra-hepatic fluoxetine concentration between 2 and 7 μmol/L. Thus PAn-mediated electrochemistry can be used to observe the monooxygenation reaction of CYP2D6. K′m is the apparent Michaelis-Menten constant and K′i is the apparent substrate inhibition constant.

Original languageEnglish (US)
Pages (from-to)929-941
Number of pages13
JournalAnalytical Letters
Volume37
Issue number5
DOIs
StatePublished - 2004

Keywords

  • CYP2D6 enzyme electrode
  • Drug metabolism biosensors
  • Fluoxetine biotransformation
  • Genetic polymorphism
  • Polyaniline-based biosensors
  • Selective serotonin reuptake inhibitor

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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