Abstract
A bioelectrode system consisting of polyaniline (PAn)-doped glassy carbon electrode (GCE) and cytochrome P4502D6 (CYP2D6) enzyme solution was used for the amperometric study of in vitro fluoxetine biotransformation. The PAn film was potentiostatically grown at +700 mV vs. Ag/AgCl (20°C) on a 0.071 cm2 GCE and used for cyclic voltammetric (CV) measurements in phosphate buffer solution (0.1 M, pH 7.5, 0.1 M KCl) of the enzyme. The response of the CYP2D6 bioelectrode to fluoxetine was consistent with uncompetitive substrate inhibition kinetics. Apparent Michaelis-Menten constant K′m of the CYP2D6 electrode was 3.7 μmol/L, which is within the intra-hepatic fluoxetine concentration between 2 and 7 μmol/L. Thus PAn-mediated electrochemistry can be used to observe the monooxygenation reaction of CYP2D6. K′m is the apparent Michaelis-Menten constant and K′i is the apparent substrate inhibition constant.
Original language | English (US) |
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Pages (from-to) | 929-941 |
Number of pages | 13 |
Journal | Analytical Letters |
Volume | 37 |
Issue number | 5 |
DOIs | |
State | Published - 2004 |
Keywords
- CYP2D6 enzyme electrode
- Drug metabolism biosensors
- Fluoxetine biotransformation
- Genetic polymorphism
- Polyaniline-based biosensors
- Selective serotonin reuptake inhibitor
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Spectroscopy
- Clinical Biochemistry
- Biochemistry, medical
- Electrochemistry