Cyclic AMP inhibits c-Ha-ras protooncogene expression, and DNA synthesis in rat aortic smooth muscle cells

D. N. Sadhu; K. S. Ramos

doi:10.1007/BF01955165

Cyclic AMP inhibits c-Ha-ras protooncogene expression, and DNA synthesis in rat aortic smooth muscle cells

D. N. Sadhu, K. S. Ramos

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Fetal bovine serum (10%) markedly increased ras transcript levels in growth-arrested (G_o-synchronized) smooth muscle cells by 8 h. This elevation was maintained for up to 18 h and returned, to pre-stimulation levels within 24 h. Challenge of quiescent cells with serum in the presence of dibetyryl cyclic AMP (1-100 μM), a growth inhibitor for smooth muscle cells, attenuated serum-induced elevation of c-Ha-ras in a dose dependent fashion and preventod progression of the cells into S phase. These results demonstrate that expression of c-Ha-ras in rat aortic smooth muscle cells is cell-cycle dependent and that cAMP prevents the induction of this protooncogene by serum.

Original language	English (US)
Pages (from-to)	567-570
Number of pages	4
Journal	Experientia
Volume	49
Issue number	6-7
DOIs	https://doi.org/10.1007/BF01955165
State	Published - Jul 1993

Keywords

aortic smooth muscle cells
c-Ha-ras
cell cycle
cyclic AMP
proliferation

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1007/BF01955165

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title = "Cyclic AMP inhibits c-Ha-ras protooncogene expression, and DNA synthesis in rat aortic smooth muscle cells",

abstract = "Fetal bovine serum (10%) markedly increased ras transcript levels in growth-arrested (Go-synchronized) smooth muscle cells by 8 h. This elevation was maintained for up to 18 h and returned, to pre-stimulation levels within 24 h. Challenge of quiescent cells with serum in the presence of dibetyryl cyclic AMP (1-100 μM), a growth inhibitor for smooth muscle cells, attenuated serum-induced elevation of c-Ha-ras in a dose dependent fashion and preventod progression of the cells into S phase. These results demonstrate that expression of c-Ha-ras in rat aortic smooth muscle cells is cell-cycle dependent and that cAMP prevents the induction of this protooncogene by serum.",

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AU - Ramos, K. S.

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N2 - Fetal bovine serum (10%) markedly increased ras transcript levels in growth-arrested (Go-synchronized) smooth muscle cells by 8 h. This elevation was maintained for up to 18 h and returned, to pre-stimulation levels within 24 h. Challenge of quiescent cells with serum in the presence of dibetyryl cyclic AMP (1-100 μM), a growth inhibitor for smooth muscle cells, attenuated serum-induced elevation of c-Ha-ras in a dose dependent fashion and preventod progression of the cells into S phase. These results demonstrate that expression of c-Ha-ras in rat aortic smooth muscle cells is cell-cycle dependent and that cAMP prevents the induction of this protooncogene by serum.

AB - Fetal bovine serum (10%) markedly increased ras transcript levels in growth-arrested (Go-synchronized) smooth muscle cells by 8 h. This elevation was maintained for up to 18 h and returned, to pre-stimulation levels within 24 h. Challenge of quiescent cells with serum in the presence of dibetyryl cyclic AMP (1-100 μM), a growth inhibitor for smooth muscle cells, attenuated serum-induced elevation of c-Ha-ras in a dose dependent fashion and preventod progression of the cells into S phase. These results demonstrate that expression of c-Ha-ras in rat aortic smooth muscle cells is cell-cycle dependent and that cAMP prevents the induction of this protooncogene by serum.

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KW - cyclic AMP

KW - proliferation

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Cyclic AMP inhibits c-Ha-ras protooncogene expression, and DNA synthesis in rat aortic smooth muscle cells

Abstract

Keywords

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