Custom selenoprotein production enabled by laboratory evolution of recoded bacterial strains

Ross Thyer, Raghav Shroff, Dustin R. Klein, Simon D'Oelsnitz, Victoria C. Cotham, Michelle Byrom, Jennifer S. Brodbelt, Andrew D. Ellington

Research output: Contribution to journalArticlepeer-review

Abstract

Incorporation of the rare amino acid selenocysteine to form diselenide bonds can improve stability and function of synthetic peptide therapeutics. However, application of this approach to recombinant proteins has been hampered by heterogeneous incorporation, low selenoprotein yields, and poor fitness of bacterial producer strains. We report the evolution of recoded Escherichia coli strains with improved fitness that are superior hosts for recombinant selenoprotein production. We apply an engineered β-lactamase containing an essential diselenide bond to enforce selenocysteine dependence during continuous evolution of recoded E. coli strains. Evolved strains maintain an expanded genetic code indefinitely. We engineer a fluorescent reporter to quantify selenocysteine incorporation in vivo and show complete decoding of UAG codons as selenocysteine. Replacement of native, labile disulfide bonds in antibody fragments with diselenide bonds vastly improves resistance to reducing conditions. Highly seleno-competent bacterial strains enable industrial-scale selenoprotein expression and unique diselenide architecture, advancing our ability to customize the selenoproteome.

Original languageEnglish (US)
Pages (from-to)624-631
Number of pages8
JournalNature Biotechnology
Volume36
Issue number7
DOIs
StatePublished - Aug 1 2018

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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