Curcumin inhibits cyclooxygenase-2 transcription in bile acid- and phorbol ester-treated human gastrointestinal epithelial cells

Fan Zhang, Nasser K. Altorki, Juan R. Mestre, Kotha Subbaramaiah, Andrew Dannenberg

Research output: Contribution to journalArticle

250 Scopus citations

Abstract

We investigated whether curcumin, a chemopreventive agent, inhibited chenodeoxycholate (CD)- or phorbol ester (PMA)-mediated induction of cyclooxygenase-2 (COX-2) in several gastrointestinal cell lines (SK-GT-4, SCC450, IEC-18 and HCA-7). Treatment with curcumin suppressed CD- and PMA-mediated induction of COX-2 protein and synthesis of prostaglandin E2. Curcumin also suppressed the induction of COX-2 mRNA by CD and PMA. Nuclear run-offs revealed increased rates of COX-2 transcription after treatment with CD or PMA and these effects were inhibited by curcumin. Treatment with CD or PMA increased binding of AP-1 to DNA. This effect was also blocked by curcumin. In addition to the above effects on gene expression, we found that curcumin directly inhibited the activity of COX-2. These data provide new insights into the anticancer properties of curcumin.

Original languageEnglish (US)
Pages (from-to)445-451
Number of pages7
JournalCarcinogenesis
Volume20
Issue number3
DOIs
StatePublished - Jan 1 1999

ASJC Scopus subject areas

  • Cancer Research

Fingerprint Dive into the research topics of 'Curcumin inhibits cyclooxygenase-2 transcription in bile acid- and phorbol ester-treated human gastrointestinal epithelial cells'. Together they form a unique fingerprint.

Cite this