Curcumin inhibits cyclooxygenase-2 transcription in bile acid- and phorbol ester-treated human gastrointestinal epithelial cells

Fan Zhang, Nasser K. Altorki, Juan R. Mestre, Kotha Subbaramaiah, Andrew J. Dannenberg

    Research output: Contribution to journalArticlepeer-review

    277 Scopus citations

    Abstract

    We investigated whether curcumin, a chemopreventive agent, inhibited chenodeoxycholate (CD)- or phorbol ester (PMA)-mediated induction of cyclooxygenase-2 (COX-2) in several gastrointestinal cell lines (SK-GT-4, SCC450, IEC-18 and HCA-7). Treatment with curcumin suppressed CD- and PMA-mediated induction of COX-2 protein and synthesis of prostaglandin E2. Curcumin also suppressed the induction of COX-2 mRNA by CD and PMA. Nuclear run-offs revealed increased rates of COX-2 transcription after treatment with CD or PMA and these effects were inhibited by curcumin. Treatment with CD or PMA increased binding of AP-1 to DNA. This effect was also blocked by curcumin. In addition to the above effects on gene expression, we found that curcumin directly inhibited the activity of COX-2. These data provide new insights into the anticancer properties of curcumin.

    Original languageEnglish (US)
    Pages (from-to)445-451
    Number of pages7
    JournalCarcinogenesis
    Volume20
    Issue number3
    DOIs
    StatePublished - 1999

    ASJC Scopus subject areas

    • Cancer Research

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