Culturing and expansion of "clinical grade" precursors cells from the fetal human central nervous system

Maurizio Gelati, Daniela Profico, Massimo Projetti-Pensi, Gianmarco Muzi, Giada Sgaravizzi, Angelo Vescovi

Research output: Chapter in Book/Report/Conference proceedingChapter

17 Scopus citations

Abstract

NSCs have been demonstrated to be very useful in grafts into the mammalian central nervous system to investigate the exploitation of NSC for the therapy of neurodegenerative disorders in animal models of neurodegenerative diseases. To push cell therapy in CNS on stage of clinical application, it is necessary to establish a continuous and standardized, clinical grade (i.e., produced following the good manufacturing practice guidelines) human neural stem cell lines. In this chapter, we illustrate some of the protocols routinely used into our GMP cell bank for the production of "clinical grade" human neural stem cell lines.

Original languageEnglish (US)
Title of host publicationNeural Progenitor Cells
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages65-77
Number of pages13
ISBN (Print)9781627035736
DOIs
StatePublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
Volume1059
ISSN (Print)1064-3745

Keywords

  • Clinical grade
  • Good manufacturing practice
  • Human central nervous system
  • Neural stem cells
  • Precursor cells
  • Therapy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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