TY - JOUR
T1 - Crystallization of ClfA and ClfB fragments
T2 - The fibrinogen-binding surface proteins of Staphylococcus aureus
AU - Deivanayagam, Champion C.S.
AU - Perkins, Samuel
AU - Danthuluri, Sita
AU - Owens, Rick T.
AU - Bice, Todd
AU - Nanavathy, Tamanna
AU - Foster, Timothy J.
AU - Höök, Magnus
AU - Narayana, Sthanam V.L.
PY - 1999/2
Y1 - 1999/2
N2 - Recombinant constructs encoding the fibrinogen-binding domains of ClfA and ClfB from Staphylococcus aureus have been crystallized. ClfA was crystallized in the orthorhombic space group P212121 with unit-cell parameters a = 39.58, b = 81.39 and c = 112.65 Å. A complete data set was recorded to 2.1 Å resolution and had a V(m) of 2.3 Å3 Da-1 with 46.5% solvent, suggesting one molecule per asymmetric unit. Co-crystals of ClfA with the 17 amino-acid C-terminal peptide of fibrinogen γ-chain diffracted to 2.1 Å resolution and had unit-cell parameters a = 39.11, b = 81.39 and c = 109.51 Å in the space group P212121. ClfB was crystallized in the tetragonal space group P41212 or P43212 with unit-cell parameters a = 96.31, b = 96.31 and c = 84.13 Å and diffracted to 2.45 Å resolution. The estimated V(m) of 2.6 Å3 Da-1 with 53% solvent indicated one molecule in the asymmetric unit.
AB - Recombinant constructs encoding the fibrinogen-binding domains of ClfA and ClfB from Staphylococcus aureus have been crystallized. ClfA was crystallized in the orthorhombic space group P212121 with unit-cell parameters a = 39.58, b = 81.39 and c = 112.65 Å. A complete data set was recorded to 2.1 Å resolution and had a V(m) of 2.3 Å3 Da-1 with 46.5% solvent, suggesting one molecule per asymmetric unit. Co-crystals of ClfA with the 17 amino-acid C-terminal peptide of fibrinogen γ-chain diffracted to 2.1 Å resolution and had unit-cell parameters a = 39.11, b = 81.39 and c = 109.51 Å in the space group P212121. ClfB was crystallized in the tetragonal space group P41212 or P43212 with unit-cell parameters a = 96.31, b = 96.31 and c = 84.13 Å and diffracted to 2.45 Å resolution. The estimated V(m) of 2.6 Å3 Da-1 with 53% solvent indicated one molecule in the asymmetric unit.
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U2 - 10.1107/S0907444998012426
DO - 10.1107/S0907444998012426
M3 - Article
C2 - 10089377
AN - SCOPUS:0033081164
SN - 0907-4449
VL - 55
SP - 554
EP - 556
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 2
ER -