TY - JOUR
T1 - Crystallization and preliminary X-ray analysis of B-domain fragments of a Staphylococcus aureus collagen-binding protein
AU - Deivanayagam, Champion C.S.
AU - Rich, Rebecca L.
AU - Danthuluri, Sita
AU - Owens, Rick T.
AU - Patti, Joseph M.
AU - Hook, Magnus
AU - DeLucas, Lawrence J.
AU - Narayana, Sthanam V.L.
PY - 1999/2/1
Y1 - 1999/2/1
N2 - Recombinant proteins of monomeric and dimeric B-domain repeats of a Staphylococcus aureus FDA 574 collagen-binding adhesin have been crystallized. The single repeat unit (B1) was crystallized in a body-centered orthorhombic lattice with a = 96.9, b = 101.3, c = 120.8 Å in either the 1222 or 1212121 space group. These crystals diffracted to 2.5 Å resolution and the calculated V(m) values of 3.2 and 2.2 Å3 Da-1 suggest the possibility of a dimer or a trimer in the asymmetric unit. The two-repeat fragment (B1B2) crystallized in the orthorhombic space group P212121 with cell dimensions a = 42.4, b = 79.4, c = 130.4 Å and diffracted to 2.3 Å resolution. For this species, the calculated V(m) value of 2.2 Å3 Da-1 indicates the presence of a monomer in the asymmetric unit.
AB - Recombinant proteins of monomeric and dimeric B-domain repeats of a Staphylococcus aureus FDA 574 collagen-binding adhesin have been crystallized. The single repeat unit (B1) was crystallized in a body-centered orthorhombic lattice with a = 96.9, b = 101.3, c = 120.8 Å in either the 1222 or 1212121 space group. These crystals diffracted to 2.5 Å resolution and the calculated V(m) values of 3.2 and 2.2 Å3 Da-1 suggest the possibility of a dimer or a trimer in the asymmetric unit. The two-repeat fragment (B1B2) crystallized in the orthorhombic space group P212121 with cell dimensions a = 42.4, b = 79.4, c = 130.4 Å and diffracted to 2.3 Å resolution. For this species, the calculated V(m) value of 2.2 Å3 Da-1 indicates the presence of a monomer in the asymmetric unit.
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U2 - 10.1107/S0907444998010051
DO - 10.1107/S0907444998010051
M3 - Article
C2 - 10089366
AN - SCOPUS:0033082043
VL - 55
SP - 525
EP - 527
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
SN - 0907-4449
IS - 2
ER -