Neural stem cells (NSCs) are, by definition, capable of unlimited self-renewal in vitro; nevertheless, there are limitations in the number of passages to avoid cell senescence and minimize the possibility of karyotype alterations. For this reason NSC cryopreservation seems to be fundamental to maintain NSC functionality in transplant experiments or to use NSCs in further derivation of neural lineages. Here, we describe a useful and detailed freezing method for NSCs.
- Human neural stem cells
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)