Cryopreservation of Human Neural Stem and Progenitor Cells

Profico Daniela Celeste; Sgaravizzi Giada; Projetti Pensi Massimo; Muzi Gianmarco; Ricciolini Claudia; Gelati Maurizio; Angelo Vescovi

doi:10.1002/9781118308295.ch6

Cryopreservation of Human Neural Stem and Progenitor Cells

Profico Daniela Celeste, Sgaravizzi Giada, Projetti Pensi Massimo, Muzi Gianmarco, Ricciolini Claudia, Gelati Maurizio, Angelo Vescovi

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Neural stem cells (NSCs) are, by definition, capable of unlimited self-renewal in vitro; nevertheless, there are limitations in the number of passages to avoid cell senescence and minimize the possibility of karyotype alterations. For this reason NSC cryopreservation seems to be fundamental to maintain NSC functionality in transplant experiments or to use NSCs in further derivation of neural lineages. Here, we describe a useful and detailed freezing method for NSCs.

Original language	English (US)
Title of host publication	Neural Stem Cell Assays
Publisher	Wiley
Pages	61-65
Number of pages	5
ISBN (Electronic)	9781118308295
ISBN (Print)	9781118308257
DOIs	https://doi.org/10.1002/9781118308295.ch6
State	Published - Jan 30 2015

Keywords

Cryopreservation
Freezing
GMP
Human neural stem cells
NSC

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1002/9781118308295.ch6

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abstract = "Neural stem cells (NSCs) are, by definition, capable of unlimited self-renewal in vitro; nevertheless, there are limitations in the number of passages to avoid cell senescence and minimize the possibility of karyotype alterations. For this reason NSC cryopreservation seems to be fundamental to maintain NSC functionality in transplant experiments or to use NSCs in further derivation of neural lineages. Here, we describe a useful and detailed freezing method for NSCs.",

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doi = "10.1002/9781118308295.ch6",

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AU - Celeste, Profico Daniela

AU - Giada, Sgaravizzi

AU - Massimo, Projetti Pensi

AU - Gianmarco, Muzi

AU - Claudia, Ricciolini

AU - Maurizio, Gelati

AU - Vescovi, Angelo

PY - 2015/1/30

Y1 - 2015/1/30

N2 - Neural stem cells (NSCs) are, by definition, capable of unlimited self-renewal in vitro; nevertheless, there are limitations in the number of passages to avoid cell senescence and minimize the possibility of karyotype alterations. For this reason NSC cryopreservation seems to be fundamental to maintain NSC functionality in transplant experiments or to use NSCs in further derivation of neural lineages. Here, we describe a useful and detailed freezing method for NSCs.

AB - Neural stem cells (NSCs) are, by definition, capable of unlimited self-renewal in vitro; nevertheless, there are limitations in the number of passages to avoid cell senescence and minimize the possibility of karyotype alterations. For this reason NSC cryopreservation seems to be fundamental to maintain NSC functionality in transplant experiments or to use NSCs in further derivation of neural lineages. Here, we describe a useful and detailed freezing method for NSCs.

KW - Cryopreservation

KW - Freezing

KW - GMP

KW - Human neural stem cells

KW - NSC

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BT - Neural Stem Cell Assays

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ER -

Cryopreservation of Human Neural Stem and Progenitor Cells

Abstract

Keywords

ASJC Scopus subject areas

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