Cryopreservation of Human Neural Stem and Progenitor Cells

Profico Daniela Celeste, Sgaravizzi Giada, Projetti Pensi Massimo, Muzi Gianmarco, Ricciolini Claudia, Gelati Maurizio, Angelo Vescovi

Research output: Chapter in Book/Report/Conference proceedingChapter


Neural stem cells (NSCs) are, by definition, capable of unlimited self-renewal in vitro; nevertheless, there are limitations in the number of passages to avoid cell senescence and minimize the possibility of karyotype alterations. For this reason NSC cryopreservation seems to be fundamental to maintain NSC functionality in transplant experiments or to use NSCs in further derivation of neural lineages. Here, we describe a useful and detailed freezing method for NSCs.

Original languageEnglish (US)
Title of host publicationNeural Stem Cell Assays
Number of pages5
ISBN (Electronic)9781118308295
ISBN (Print)9781118308257
StatePublished - Jan 30 2015


  • Cryopreservation
  • Freezing
  • GMP
  • Human neural stem cells
  • NSC

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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