Cryopreservation of Human Neural Stem and Progenitor Cells

Profico Daniela Celeste, Sgaravizzi Giada, Projetti Pensi Massimo, Muzi Gianmarco, Ricciolini Claudia, Gelati Maurizio, Angelo Vescovi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Neural stem cells (NSCs) are, by definition, capable of unlimited self-renewal in vitro; nevertheless, there are limitations in the number of passages to avoid cell senescence and minimize the possibility of karyotype alterations. For this reason NSC cryopreservation seems to be fundamental to maintain NSC functionality in transplant experiments or to use NSCs in further derivation of neural lineages. Here, we describe a useful and detailed freezing method for NSCs.

Original languageEnglish (US)
Title of host publicationNeural Stem Cell Assays
PublisherWiley
Pages61-65
Number of pages5
ISBN (Electronic)9781118308295
ISBN (Print)9781118308257
DOIs
StatePublished - Jan 30 2015

Keywords

  • Cryopreservation
  • Freezing
  • GMP
  • Human neural stem cells
  • NSC

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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