Abstract
Neural stem cells (NSCs) are, by definition, capable of unlimited self-renewal in vitro; nevertheless, there are limitations in the number of passages to avoid cell senescence and minimize the possibility of karyotype alterations. For this reason NSC cryopreservation seems to be fundamental to maintain NSC functionality in transplant experiments or to use NSCs in further derivation of neural lineages. Here, we describe a useful and detailed freezing method for NSCs.
Original language | English (US) |
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Title of host publication | Neural Stem Cell Assays |
Publisher | Wiley |
Pages | 61-65 |
Number of pages | 5 |
ISBN (Electronic) | 9781118308295 |
ISBN (Print) | 9781118308257 |
DOIs | |
State | Published - Jan 30 2015 |
Keywords
- Cryopreservation
- Freezing
- GMP
- Human neural stem cells
- NSC
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)