CRR9/CLPTM1L regulates cell survival signaling and is required for ras transformation and lung tumorigenesis

Michael A. James, Haris G. Vikis, Everett Tate, Amy L. Rymaszewski, Ming You

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

The transmembrane protein CLPTM1L is overexpressed in non-small cell lung cancer, where it protects tumor cells from genotoxic apoptosis. Here, we show that RNA interference-mediated blockade of CLPTM1L inhibits K-Ras-induced lung tumorigenesis. CLPTM1L expression was required in vitro for morphologic transformation by H-RasV12 or K-RasV12, anchorage-independent growth, and survival of anoikis of lung tumor cells. Mechanistic investigations indicated that CLPTM1L interacts with phosphoinositide 3-kinase and is essential for Ras-induced AKT phosphorylation. Furthermore that the anti-apoptotic protein Bcl-xL is regulated by CLPTM1L independently of AKT activation. Constitutive activation of AKT or Bcl-xL rescued the transformed phenotype in CLPTM1L-depleted cells. The CLPTM1L gene lies within a cancer susceptibility locus at chromosome 5p15.33 defined by genome-wide association studies. The risk genotype at the CLPTM1L locus was associated with high expression of CLPTM1L in normal lung tissue, suggesting that cis-regulation of CLPTM1L may contribute to lung cancer risk. Taken together, our results establish a protumorigenic role for CLPTM1L that is critical for Ras-driven lung cancers, with potential implications for therapy and chemosensitization.

Original languageEnglish (US)
Pages (from-to)1116-1127
Number of pages12
JournalCancer research
Volume74
Issue number4
DOIs
StatePublished - Feb 15 2014

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint

Dive into the research topics of 'CRR9/CLPTM1L regulates cell survival signaling and is required for ras transformation and lung tumorigenesis'. Together they form a unique fingerprint.

Cite this