CRISPR-Cas12a Coupled with Platinum Nanoreporter for Visual Quantification of SNVs on a Volumetric Bar-Chart Chip

Ning Shao, Xin Han, Yanni Song, Pengchao Zhang, Lidong Qin

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Methods that can detect and quantify single nucleotide variations (SNVs)/single nucleotide polymorphisms (SNPs) are greatly needed in the bioanalytical measurement of gene mutations and polymorphisms. Herein a visual and instrument-free SNV quantification platform is developed. Platinum nanoparticles tethered to magnetic beads by single-stranded DNAs are designed as quantitative readout reporters for a CRISPR-Cas12a nucleic acid detection system. The integration of platinum nanoreporter and CRISPR-Cas system with a volumetric bar-chart chip realizes the volumetric quantification of nucleic acids. This platform enables quantification of multiple cancer mutations in pure DNA samples and mock cell-free DNA samples in serum, with allelic fractions as low as 0.01%. This platform could have great potential in the quantification of SNVs/SNPs as well as other types of nucleic acid targets at the point of care.

Original languageEnglish (US)
Pages (from-to)12384-12391
Number of pages8
JournalAnalytical Chemistry
Volume91
Issue number19
DOIs
StatePublished - Oct 1 2019

ASJC Scopus subject areas

  • Analytical Chemistry

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