TY - JOUR
T1 - Creation of a bacterial cell controlled by a chemically synthesized genome
AU - Gibson, Daniel G.
AU - Glass, John I.
AU - Lartigue, Carole
AU - Noskov, Vladimir N.
AU - Chuang, Ray Yuan
AU - Algire, Mikkel A.
AU - Benders, Gwynedd A.
AU - Montague, Michael G.
AU - Ma, Li
AU - Moodie, Monzia M.
AU - Merryman, Chuck
AU - Vashee, Sanjay
AU - Krishnakumar, Radha
AU - Assad-Garcia, Nacyra
AU - Andrews-Pfannkoch, Cynthia
AU - Denisova, Evgeniya A.
AU - Young, Lei
AU - Qi, Zhi Ng
AU - Segall-Shapiro, Thomas H.
AU - Calvey, Christopher H.
AU - Parmar, Prashanth P.
AU - Hutchison, Clyde A.
AU - Smith, Hamilton O.
AU - Venter, J. Craig
PY - 2010/7/2
Y1 - 2010/7/2
N2 - We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.
AB - We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.
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U2 - 10.1126/science.1190719
DO - 10.1126/science.1190719
M3 - Article
C2 - 20488990
AN - SCOPUS:77953584054
SN - 0036-8075
VL - 329
SP - 52
EP - 56
JO - Science
JF - Science
IS - 5987
ER -