Creation of a bacterial cell controlled by a chemically synthesized genome

Daniel G. Gibson; John I. Glass; Carole Lartigue; Vladimir N. Noskov; Ray Yuan Chuang; Mikkel A. Algire; Gwynedd A. Benders; Michael G. Montague; Li Ma; Monzia M. Moodie; Chuck Merryman; Sanjay Vashee; Radha Krishnakumar; Nacyra Assad-Garcia; Cynthia Andrews-Pfannkoch; Evgeniya A. Denisova; Lei Young; Zhi Ng Qi; Thomas H. Segall-Shapiro; Christopher H. Calvey; Prashanth P. Parmar; Clyde A. Hutchison; Hamilton O. Smith; J. Craig Venter

doi:10.1126/science.1190719

Creation of a bacterial cell controlled by a chemically synthesized genome

Daniel G. Gibson, John I. Glass, Carole Lartigue, Vladimir N. Noskov, Ray Yuan Chuang, Mikkel A. Algire, Gwynedd A. Benders, Michael G. Montague, Li Ma, Monzia M. Moodie, Chuck Merryman, Sanjay Vashee, Radha Krishnakumar, Nacyra Assad-Garcia, Cynthia Andrews-Pfannkoch, Evgeniya A. Denisova, Lei Young, Zhi Ng Qi, Thomas H. Segall-Shapiro, Christopher H. CalveyPrashanth P. Parmar, Clyde A. Hutchison, Hamilton O. Smith, J. Craig Venter

Research output: Contribution to journal › Article › peer-review

1673 Scopus citations

Abstract

We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.

Original language	English (US)
Pages (from-to)	52-56
Number of pages	5
Journal	Science
Volume	329
Issue number	5987
DOIs	https://doi.org/10.1126/science.1190719
State	Published - Jul 2 2010

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Gibson, D. G., Glass, J. I., Lartigue, C., Noskov, V. N., Chuang, R. Y., Algire, M. A., Benders, G. A., Montague, M. G., Ma, L., Moodie, M. M., Merryman, C., Vashee, S., Krishnakumar, R., Assad-Garcia, N., Andrews-Pfannkoch, C., Denisova, E. A., Young, L., Qi, Z. N., Segall-Shapiro, T. H., ... Venter, J. C. (2010). Creation of a bacterial cell controlled by a chemically synthesized genome. Science, 329(5987), 52-56. https://doi.org/10.1126/science.1190719

Creation of a bacterial cell controlled by a chemically synthesized genome. / Gibson, Daniel G.; Glass, John I.; Lartigue, Carole; Noskov, Vladimir N.; Chuang, Ray Yuan; Algire, Mikkel A.; Benders, Gwynedd A.; Montague, Michael G.; Ma, Li; Moodie, Monzia M.; Merryman, Chuck; Vashee, Sanjay; Krishnakumar, Radha; Assad-Garcia, Nacyra; Andrews-Pfannkoch, Cynthia; Denisova, Evgeniya A.; Young, Lei; Qi, Zhi Ng; Segall-Shapiro, Thomas H.; Calvey, Christopher H.; Parmar, Prashanth P.; Hutchison, Clyde A.; Smith, Hamilton O.; Venter, J. Craig.

In: Science, Vol. 329, No. 5987, 02.07.2010, p. 52-56.

Research output: Contribution to journal › Article › peer-review

Gibson, DG, Glass, JI, Lartigue, C, Noskov, VN, Chuang, RY, Algire, MA, Benders, GA, Montague, MG, Ma, L, Moodie, MM, Merryman, C, Vashee, S, Krishnakumar, R, Assad-Garcia, N, Andrews-Pfannkoch, C, Denisova, EA, Young, L, Qi, ZN, Segall-Shapiro, TH, Calvey, CH, Parmar, PP, Hutchison, CA, Smith, HO & Venter, JC 2010, 'Creation of a bacterial cell controlled by a chemically synthesized genome', Science, vol. 329, no. 5987, pp. 52-56. https://doi.org/10.1126/science.1190719

Gibson, Daniel G. ; Glass, John I. ; Lartigue, Carole ; Noskov, Vladimir N. ; Chuang, Ray Yuan ; Algire, Mikkel A. ; Benders, Gwynedd A. ; Montague, Michael G. ; Ma, Li ; Moodie, Monzia M. ; Merryman, Chuck ; Vashee, Sanjay ; Krishnakumar, Radha ; Assad-Garcia, Nacyra ; Andrews-Pfannkoch, Cynthia ; Denisova, Evgeniya A. ; Young, Lei ; Qi, Zhi Ng ; Segall-Shapiro, Thomas H. ; Calvey, Christopher H. ; Parmar, Prashanth P. ; Hutchison, Clyde A. ; Smith, Hamilton O. ; Venter, J. Craig. / Creation of a bacterial cell controlled by a chemically synthesized genome. In: Science. 2010 ; Vol. 329, No. 5987. pp. 52-56.

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abstract = "We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including {"}watermark{"} sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.",

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AU - Algire, Mikkel A.

AU - Benders, Gwynedd A.

AU - Montague, Michael G.

AU - Ma, Li

AU - Moodie, Monzia M.

AU - Merryman, Chuck

AU - Vashee, Sanjay

AU - Krishnakumar, Radha

AU - Assad-Garcia, Nacyra

AU - Andrews-Pfannkoch, Cynthia

AU - Denisova, Evgeniya A.

AU - Young, Lei

AU - Qi, Zhi Ng

AU - Segall-Shapiro, Thomas H.

AU - Calvey, Christopher H.

AU - Parmar, Prashanth P.

AU - Hutchison, Clyde A.

AU - Smith, Hamilton O.

AU - Venter, J. Craig

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Creation of a bacterial cell controlled by a chemically synthesized genome

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