Cre-mediated cerebellum- and hippocampus-restricted gene mutation in mouse brain

Huailian Guo, Chengjian Mao, Xiao Lu Jin, Hong Wang, Yen Te Tu, Prachee Pradeep Avasthi, Yuqing Li

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Using the phage P1-drived Cre/loxP recombination system, we have created a line of cre-transgenic mice in which the Cre-mediated gene deletion is restricted to granule cells of cerebellum and dentate gyrus of hippocampus. Low levels of deletion were also present in pyramidal cells of hippocampal CA1 and CA3 fields. The Cre/loxP recombination occurred prenatally. The recombination efficiencies in the granular layer of the cerebellum, the granular layer of the dentate gyrus, and the CA1 and CA3 pyramidal cells of the hippocampus were 34.0%, 23.1%, 3.0%, and 9.8%, respectively. This line of cre-transgenic mice should be conducive to studies of the effect of a gene mutation upon brain development and plasticity.

Original languageEnglish (US)
Pages (from-to)149-154
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume269
Issue number1
DOIs
StatePublished - Mar 5 2000

Keywords

  • CA1
  • CA3
  • Cerebellum
  • Cre/loxP
  • Dentate gyrus
  • Emx1
  • Granule cells
  • Hippocampus
  • Transgenic mice
  • β-galactosidase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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