@article{80b2b117cead406db60d75aacd098237,
title = "Cre-mediated cerebellum- and hippocampus-restricted gene mutation in mouse brain",
abstract = "Using the phage P1-drived Cre/loxP recombination system, we have created a line of cre-transgenic mice in which the Cre-mediated gene deletion is restricted to granule cells of cerebellum and dentate gyrus of hippocampus. Low levels of deletion were also present in pyramidal cells of hippocampal CA1 and CA3 fields. The Cre/loxP recombination occurred prenatally. The recombination efficiencies in the granular layer of the cerebellum, the granular layer of the dentate gyrus, and the CA1 and CA3 pyramidal cells of the hippocampus were 34.0%, 23.1%, 3.0%, and 9.8%, respectively. This line of cre-transgenic mice should be conducive to studies of the effect of a gene mutation upon brain development and plasticity.",
keywords = "CA1, CA3, Cerebellum, Cre/loxP, Dentate gyrus, Emx1, Granule cells, Hippocampus, Transgenic mice, β-galactosidase",
author = "Huailian Guo and Chengjian Mao and Jin, {Xiao Lu} and Hong Wang and Tu, {Yen Te} and Avasthi, {Prachee Pradeep} and Yuqing Li",
note = "Funding Information: We thank Dr. Anna Klintsova for advice on using Stereology methods; Drs. J. Murray, N. Gaiano, K. Kawakami, P. Mombaerts, Y. Wang, D. Anderson, C. Lobe, A. Nagy, and P. Soriano for their gifts of various reagents; Drs. A. Nardulli, W. Greenough, and J. Sweedler for the use of their equipment; Dr. M. Wheeler for the making of the transgenic mice; Dr. Guo-Ping Fan for the advice on the interpretation of the deletion results; Dr. S. Hong for critical reading of the manuscript; and the members of the Li lab for helpful discussion and encouragement. Weimin Zen and Kim Chaney performed part of the experiments for the initial characterization of the transgenic mice. This work was supported by grants from NSF, NIH, and by a startup fund from the State of Illinois, Beckman Institute, and the Lucille P. Markey Charitable Trust.",
year = "2000",
month = mar,
day = "5",
doi = "10.1006/bbrc.2000.2263",
language = "English (US)",
volume = "269",
pages = "149--154",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press",
number = "1",
}