TY - JOUR
T1 - CORRIGENDUM
T2 - GATA3 as a master regulator for interactions of tumor-associated macrophages with high-grade serous ovarian carcinoma
AU - El-Arabey, Amr Ahmed
AU - Denizli, Merve
AU - Kanlikilicer, Pinar
AU - Bayraktar, Recep
AU - Ivan, Cristina
AU - Rashed, Mohammed
AU - Kabil, Nashwa
AU - Ozpolat, Bulent
AU - Calin, George A.
AU - Salama, Salama Abdou
AU - Abd-Allah, Adel Rashad
AU - Sood, Anil K.
AU - Lopez-Berestein, Gabriel
N1 - Publisher Copyright:
© 2020
PY - 2022/1
Y1 - 2022/1
N2 - The authors apologize for the error in Fig. 2E- The Figure legend should have made it clear that M0 macrophages: the top panel was a low magnification and the mid panel was a higher magnification of the same cell. There was no intent of duplication. Fig. 2 corrected legend. Fig. 2 Macrophage and exosome characterization. (A) Morphology of undifferentiated monocytes at day 0, showing a cell diameter of <30 μm under 3D atomic force microscopy (AFM). (B) Morphology of M0 macrophages after 3 days of treatment, showing a cell diameter of >30 μm under 3D AFM. (C) Morphology of M2 macrophages after 24 h of treatment with IL-4 and IL-13, showing a cell diameter of >100 μm under 3D AFM. (D, E) Characteristic morphology of M2 macrophages compared with M0 macrophages: cell elongation to modulate macrophage phenotype polarization (AFM and 3D optical microscopy); E) M0 macrophages: top panel- low magnification and mid panel- higher magnification of the same cell (F) Immunoblotting to validate the macrophage markers CD206, CD68, and CD163 in differentiated THP-1 cells and undifferentiated THP-1 cells. (G) Flow cytometry analysis to verify differential expression of M2 markers CD163 and CD206 between differentiated and undifferentiated THP-1 cells (P < .05). (H) Quantification of isolated exosomes from tumor-associated macrophages (Nano sight).
AB - The authors apologize for the error in Fig. 2E- The Figure legend should have made it clear that M0 macrophages: the top panel was a low magnification and the mid panel was a higher magnification of the same cell. There was no intent of duplication. Fig. 2 corrected legend. Fig. 2 Macrophage and exosome characterization. (A) Morphology of undifferentiated monocytes at day 0, showing a cell diameter of <30 μm under 3D atomic force microscopy (AFM). (B) Morphology of M0 macrophages after 3 days of treatment, showing a cell diameter of >30 μm under 3D AFM. (C) Morphology of M2 macrophages after 24 h of treatment with IL-4 and IL-13, showing a cell diameter of >100 μm under 3D AFM. (D, E) Characteristic morphology of M2 macrophages compared with M0 macrophages: cell elongation to modulate macrophage phenotype polarization (AFM and 3D optical microscopy); E) M0 macrophages: top panel- low magnification and mid panel- higher magnification of the same cell (F) Immunoblotting to validate the macrophage markers CD206, CD68, and CD163 in differentiated THP-1 cells and undifferentiated THP-1 cells. (G) Flow cytometry analysis to verify differential expression of M2 markers CD163 and CD206 between differentiated and undifferentiated THP-1 cells (P < .05). (H) Quantification of isolated exosomes from tumor-associated macrophages (Nano sight).
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U2 - 10.1016/j.cellsig.2021.110147
DO - 10.1016/j.cellsig.2021.110147
M3 - Article
C2 - 34772589
AN - SCOPUS:85118893292
SN - 0898-6568
VL - 89
SP - 110147
JO - Cellular Signalling
JF - Cellular Signalling
M1 - 110147
ER -