The influence of crossmatch test sensitivity on the outcome of renal transplantation is not clearly established. However, acute irreversible rejection is the most frequent cause of renal allograft failure; undetected recipient preimmunization may be a contributing factor. Thus, there has been a continued effort to develop more sensitive crossmatch techniques. Recently, Garavoy et al have described a technique which detects the binding of antibodies in recipient sera to donor lymphocytes using an immunofluorescent reagent detectable with the fluorescence activated cell sorter. Preliminary results from several labs including our own have suggested that immunofluorescent flow cytometric crossmatches are more sensitive than standard serological techniques which depend on complement dependent cytotoxicity and may be useful in predicting the outcome of renal transplantation. We have used the T cell flow cytometric (FC) immunofluorescence crossmatch to identify what we think is a high risk population of renal transplant recipients.
|Original language||English (US)|
|Number of pages||3|
|State||Published - Jan 1 1986|
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