TY - JOUR
T1 - Correlation between bioassay-derived P4501A1 induction activity and chemical analysis of clam (Laternula elliptica) extracts from McMurdo Sound, Antarctica
AU - McDonald, Susanne J.
AU - Kennicutt, Mahlon C.
AU - Sericano, Jose
AU - Wade, Terry L.
AU - Liu, Hong
AU - Safe, Stephen H.
N1 - Funding Information:
Samples were collected by John Oliver and coworkers. The financial assistance of the National Institut, Health (P42-ES04917), the Texas Agricultural Experiment Station, the National Science Foundation Polar Prog (OPP-9022346) and the National Oceanographic and Atmospheric Status and Trends Contract (50-D6NC-5-00 is gratefully acknowledged. S. Safe is a Burroughs Wellcome Toxicology Scholar.
PY - 1994/6
Y1 - 1994/6
N2 - Variable levels of halogenated aromatic hydrocarbons were measured in clams (Laternula elliptica) collected from McMurdo Sound, Antarctica. Clams collected in and near Winter Quarters Bay contained high levels of organochlorine compounds, particularly polychlorinated biphenyls (PCBs). A strong gradient has been documented in Winter Quarters Bay that been linked to human activities at McMurdo Station. The activity of clam extracts as inducers of P4501A1-dependent ethoxyresorufin O-deethylase (EROD) activity was determined using in vitro bioassays utilizing rat hepatoma H4IIE cells. The extracts which exhibited the highest induction activities were those derived from clams collected in contaminated areas. Additionally, there was an excellent linear correlation between induced EROD activity versus total PCB levels (r2=0.96). The complimentary nature of both the analytical and bioanalytical data confirms the utility of the latter assay and provides a method for estimating the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxic equivalents in extracts from marine biota.
AB - Variable levels of halogenated aromatic hydrocarbons were measured in clams (Laternula elliptica) collected from McMurdo Sound, Antarctica. Clams collected in and near Winter Quarters Bay contained high levels of organochlorine compounds, particularly polychlorinated biphenyls (PCBs). A strong gradient has been documented in Winter Quarters Bay that been linked to human activities at McMurdo Station. The activity of clam extracts as inducers of P4501A1-dependent ethoxyresorufin O-deethylase (EROD) activity was determined using in vitro bioassays utilizing rat hepatoma H4IIE cells. The extracts which exhibited the highest induction activities were those derived from clams collected in contaminated areas. Additionally, there was an excellent linear correlation between induced EROD activity versus total PCB levels (r2=0.96). The complimentary nature of both the analytical and bioanalytical data confirms the utility of the latter assay and provides a method for estimating the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxic equivalents in extracts from marine biota.
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U2 - 10.1016/0045-6535(94)90189-9
DO - 10.1016/0045-6535(94)90189-9
M3 - Article
AN - SCOPUS:0028337399
SN - 0045-6535
VL - 28
SP - 2237
EP - 2248
JO - Chemosphere
JF - Chemosphere
IS - 12
ER -