Correlation between bioassay-derived P4501A1 induction activity and chemical analysis of clam (Laternula elliptica) extracts from McMurdo Sound, Antarctica

Susanne J. McDonald, Mahlon C. Kennicutt, Jose Sericano, Terry L. Wade, Hong Liu, Stephen H. Safe

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Variable levels of halogenated aromatic hydrocarbons were measured in clams (Laternula elliptica) collected from McMurdo Sound, Antarctica. Clams collected in and near Winter Quarters Bay contained high levels of organochlorine compounds, particularly polychlorinated biphenyls (PCBs). A strong gradient has been documented in Winter Quarters Bay that been linked to human activities at McMurdo Station. The activity of clam extracts as inducers of P4501A1-dependent ethoxyresorufin O-deethylase (EROD) activity was determined using in vitro bioassays utilizing rat hepatoma H4IIE cells. The extracts which exhibited the highest induction activities were those derived from clams collected in contaminated areas. Additionally, there was an excellent linear correlation between induced EROD activity versus total PCB levels (r2=0.96). The complimentary nature of both the analytical and bioanalytical data confirms the utility of the latter assay and provides a method for estimating the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxic equivalents in extracts from marine biota.

Original languageEnglish (US)
Pages (from-to)2237-2248
Number of pages12
JournalChemosphere
Volume28
Issue number12
DOIs
StatePublished - Jun 1994
Externally publishedYes

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Correlation between bioassay-derived P4501A1 induction activity and chemical analysis of clam (Laternula elliptica) extracts from McMurdo Sound, Antarctica'. Together they form a unique fingerprint.

Cite this