Corrected and republished from: BCL11A is a critical component of a transcriptional network that activates RAG expression and V(D)J recombination

Baeck Seung Lee, Bum Kyu Lee, Vishwanath R. Iyer, Barry P. Sleckman, Arthur L. Shaffer, Gregory C. Ippolito, Haley O. Tucker, Joseph D. Dekker

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Recombination activating gene 1 (RAG1) and RAG2 are critical enzymes for initiating variable-diversity-joining [V(D)J] segment recombination, an essential process for antigen receptor expression and lymphocyte development. The BCL11A transcription factor is required for B cell and plasmacytoid dendritic cell (pDC) development, but its molecular function(s) in early B cell fate specification and commitment is unknown. We show here that the major B cell isoform, BCL11A-XL, binds directly to the RAG1 promoter as well as directly to regulatory regions of transcription factors previously implicated in both B cell and pDC development to activate RAG1 and RAG2 gene transcription in pro- and pre-B cells. We employed BCL11A overexpression with recombination substrates to demonstrate direct consequences of BCL11A/RAG modulation on V(D)J recombination. We conclude that BCL11A is a critical component of a transcriptional network that regulates B cell fate by controlling V(D)J recombination.

Original languageEnglish (US)
Article numbere00362-17
JournalMolecular and Cellular Biology
Volume38
Issue number1
DOIs
StatePublished - Jan 1 2018

Keywords

  • B cell development
  • Bcl11a
  • Immunology
  • RAG
  • V(D)J

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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